Yin Benlan, Zhang Yujiao, Wei Xiaohong, Pang Chunrong, Hou Ting, Yang Chao, Ning Yuzhi, Fu Xiaodong
Department of Obstetrics, The Affiliated Hospital of Southwest Medical University, No.8 Kangcheng Road, Luzhou, 646000, Sichuan Province, China.
Department of Obstetrics, Guizhou Provincial People's Hospital, No.83 Zhongshan East Road, Guiyang, 550000, Guizhou Province, China.
Sci Rep. 2021 Apr 12;11(1):7955. doi: 10.1038/s41598-021-87146-6.
The aim of this study is to establish the expression of ATP-sensitive potassium channels(KATP) in human umbilical arteries with severe pre-eclampsia. Real-time quantitative PCR and western blotting were used to detect the mRNA and protein expression levels of KATP channel subunits Kir6.1 and SUR2B in human umbilical arteries from normal pregnant and those with severe pre-eclampsia, early onset severe pre-eclampsia and late onset severe pre-eclampsia. The mRNA and protein levels of SUR2B in the severe pre-eclampsia group were lower than those in the normal group (P < 0.001), and the expression of Kir6.1 was not statistically significant between the two groups (P > 0.05). The mRNA and protein levels of SUR2B in early onset severe pre-eclampsia group were lower than those in late onset severe pre-eclampsia group (P < 0.001). There was no significant difference in expression of Kir6.1 between the two groups (P > 0.05). The mRNA and protein expression levels of SUR2B in pregnant women with severe pre-eclampsia were lower than those in normal pregnant women, suggesting that the expression of the SUR2B of the KATP channel may be related to the occurrence and development of severe pre-eclampsia. Compared with late onset severe pre-eclampsia, the mRNA and protein expression levels of SUR2B were lower in the umbilical arteries of women with early onset severe pre-eclampsia, suggesting that the occurrence time of severe pre-eclampsia may be related to the extent reduced expression of the SUR2B of the KATP channel.
本研究旨在确定三磷酸腺苷敏感性钾通道（KATP）在重度子痫前期患者脐动脉中的表达情况。采用实时定量聚合酶链反应和蛋白质免疫印迹法检测正常妊娠孕妇、重度子痫前期患者、早发型重度子痫前期患者及晚发型重度子痫前期患者脐动脉中KATP通道亚基Kir6.1和SUR2B的信使核糖核酸（mRNA）和蛋白表达水平。重度子痫前期组SUR2B的mRNA和蛋白水平低于正常组（P＜0.001），两组间Kir6.1的表达差异无统计学意义（P＞0.05）。早发型重度子痫前期组SUR2B的mRNA和蛋白水平低于晚发型重度子痫前期组（P＜0.001）。两组间Kir6.1的表达差异无统计学意义（P＞0.05）。重度子痫前期孕妇SUR2B的mRNA和蛋白表达水平低于正常妊娠孕妇，提示KATP通道SUR2B的表达可能与重度子痫前期的发生发展有关。与晚发型重度子痫前期相比，早发型重度子痫前期患者脐动脉中SUR2B的mRNA和蛋白表达水平更低，提示重度子痫前期的发病时间可能与KATP通道SUR2B表达降低的程度有关。
