Anderson P, Vaughan A T, Varley N R
Department of Immunology, University of Birmingham Medical School, U.K.
Int J Rad Appl Instrum B. 1988;15(3):293-7. doi: 10.1016/0883-2897(88)90109-2.
We have investigated the direct labeling of antibodies with gold isotopes as an alternative to iodine and chelated metals for antibody guided therapy. Both 199Au and 195Au complex anions in citrate-buffered solutions (pH 3.8-7.2) are rapidly bound to sheep polyclonal anti-CEA with high efficiency. After purification by gel filtration, the antibody retained 80% of the bound gold for upto 6 days at 4 degrees C and was reactive with its target antigen in a solid phase assay. Incubation with the gold binding substance D-penicillamine reduced labeling efficiency and stability.
我们研究了用金同位素直接标记抗体，以此作为碘和螯合金属用于抗体导向治疗的替代方法。在柠檬酸盐缓冲溶液（pH 3.8 - 7.2）中，¹⁹⁹Au和¹⁹⁵Au的复合阴离子都能快速且高效地与羊抗癌胚抗原多克隆抗体结合。经凝胶过滤纯化后，该抗体在4℃下可在长达6天的时间内保留80%结合的金，并且在固相分析中能与靶抗原发生反应。与金结合物质D - 青霉胺一起孵育会降低标记效率和稳定性。
