Yu Si-Yuan, Ruan Yi-Fan, Liu Yi-Li, Han De-Man, Zhou Hong, Zhao Wei-Wei, Jiang Dechen, Xu Jing-Juan, Chen Hong-Yuan
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
Engineering Research Center of Recycling & Comprehensive Utilization of Pharmaceutical and Chemical Waste of Zhejiang Province, Taizhou University, Jiaojiang 318000, China.
ACS Sens. 2021 Apr 23;6(4):1529-1535. doi: 10.1021/acssensors.1c00463. Epub 2021 Apr 13.
Emerging nanopipette tools have demonstrated substantial potential for advanced single-cell analysis, which plays vital roles from fundamental cellular biology to biomedical diagnostics. Highly recyclable nanopipettes with easy and quick regeneration are of special interest for precise and multiple measurements. However, existing recycle strategies are generally plagued by operational complexity and limited efficiency. Light, acting in a noncontact way, should be the ideal external stimulus to address this issue. Herein, we present the photocontrolled nanopipette capable of probing cellular adenosine triphosphate (ATP) gradient at single-cell level with good sensitivity, selectivity, and reversibility, which stems from the use of ATP-specific azobenzene (Azo)-incorporated DNA aptamer strands (AIDAS) and thereby the sensible transduction of variable nanopore size by the ionic currents passing through the aperture. Photoisomerized conformational change of the AIDAS by alternative UV/vis light stimulation ensures its noninvasive regeneration and repeated detection. Inducement and inhibition of the cellular ATP could also be probed by this nanosensor.
新兴的纳米吸管工具已显示出在先进单细胞分析方面的巨大潜力，这在从基础细胞生物学到生物医学诊断等领域都发挥着至关重要的作用。具有易于快速再生的高度可回收纳米吸管对于精确和多次测量尤为重要。然而，现有的回收策略通常受到操作复杂性和效率有限的困扰。以非接触方式作用的光应该是解决这个问题的理想外部刺激。在此，我们展示了一种光控纳米吸管，它能够在单细胞水平上探测细胞三磷酸腺苷（ATP）梯度，具有良好的灵敏度、选择性和可逆性，这源于使用了结合有ATP特异性偶氮苯（Azo）的DNA适配体链（AIDAS），从而通过穿过孔径的离子电流实现可变纳米孔尺寸的灵敏转导。通过交替的紫外/可见光刺激使AIDAS发生光异构化构象变化，确保了其无创再生和重复检测。这种纳米传感器还可以探测细胞ATP的诱导和抑制。
