Hoff Kendall, Ding Xun, Carter Lucas, Duque John, Lin Ju-Yu, Dung Samantha, Singh Priyanka, Sun Jiayi, Crnogorac Filip, Swaminathan Radha, Alden Emily N, Zhu Xuechen, Shimada Ryota, Posavi Marijan, Hull Noah, Dinwiddie Darrell, Halasz Adam M, McGall Glenn, Zhou Wei, Edwards Jeremy S
Centrillion Technologies, Palo Alto, California 94303, United States.
Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, New Mexico 87131, United States.
Langmuir. 2021 Apr 27;37(16):4763-4771. doi: 10.1021/acs.langmuir.0c02927. Epub 2021 Apr 13.
SARS-CoV-2 has infected over 128 million people worldwide, and until a vaccine is developed and widely disseminated, vigilant testing and contact tracing are the most effective ways to slow the spread of COVID-19. Typical clinical testing only confirms the presence or absence of the virus, but rather, a simple and rapid testing procedure that sequences the entire genome would be impactful and allow for tracing the spread of the virus and variants, as well as the appearance of new variants. However, traditional short read sequencing methods are time consuming and expensive. Herein, we describe a tiled genome array that we developed for rapid and inexpensive full viral genome resequencing, and we have applied our SARS-CoV-2-specific genome tiling array to rapidly and accurately resequence the viral genome from eight clinical samples. We have resequenced eight samples acquired from patients in Wyoming that tested positive for SARS-CoV-2. We were ultimately able to sequence over 95% of the genome of each sample with greater than 99.9% average accuracy.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）已在全球感染了超过1.28亿人，在疫苗研发并广泛普及之前，严格的检测和接触者追踪是减缓新冠病毒传播的最有效方法。典型的临床检测仅能确认病毒的存在与否，而一种对整个基因组进行测序的简单快速检测程序将具有重要意义，能够追踪病毒及其变种的传播情况以及新变种的出现。然而，传统的短读长测序方法既耗时又昂贵。在此，我们描述了一种为快速且低成本的全病毒基因组重测序而开发的平铺基因组阵列，并且我们已将我们的SARS-CoV-2特异性基因组平铺阵列应用于对八个临床样本中的病毒基因组进行快速准确的重测序。我们对从怀俄明州检测出SARS-CoV-2呈阳性的患者身上采集的八个样本进行了重测序。最终，我们能够对每个样本超过95%的基因组进行测序，平均准确率超过99.9%。
