Xia Yin-He, Shi Zi-Cong, Wang Xin-Wei, Li Yong-Tao, Wang Zeng, Chang Hong-Tao, Liu Hong-Ying, Chen Lu, Wang Chuan-Qing, Yang Xia
College of Veterinary Medicine, Henan Agricultural University, China.
College of Veterinary Medicine, Henan Agricultural University, China.
Mol Cell Probes. 2021 Jun;57:101730. doi: 10.1016/j.mcp.2021.101730. Epub 2021 Apr 14.
Getah virus (GETV), a mosquito-borne virus belonging to the Alphavirus genus of family Togaviridae, has become increasingly problematic, which poses a huge threat to the safety of animals and public health. In order to detect GETV quickly and accurately, we have developed a SYBR Green I real-time quantitative reverse transcription PCR (RT-qPCR) assay for GETV with the detection limit of 66 copies/μL, excellent correlation coefficient (R) of 0.9975, and amplification efficiency (E) of 98.90%, the target selected was the non-structural protein 3 of GETV. The sensitivity of it was higher than that of ordinary RT-PCR by 1000 folds, and the inter-assay and intra-assay CV values were all less than 0.99%. The newly developed RT-qPCR assay exhibited good sensitivity and reproducibility, which will provide technical support for the reliable and specific rapid diagnosis, and quantitative analysis of GETV infection.
盖塔病毒（GETV）是一种由蚊子传播的病毒，属于披膜病毒科甲病毒属，其问题日益严重，对动物安全和公众健康构成巨大威胁。为了快速准确地检测GETV，我们开发了一种针对GETV的SYBR Green I实时定量逆转录PCR（RT-qPCR）检测方法，检测限为66拷贝/μL，具有0.9975的出色相关系数（R）和98.90%的扩增效率（E），所选靶标为GETV的非结构蛋白3。其灵敏度比普通RT-PCR高1000倍，批间和批内变异系数（CV）值均小于0.99%。新开发的RT-qPCR检测方法具有良好的灵敏度和重复性，将为GETV感染的可靠、特异性快速诊断及定量分析提供技术支持。
