Li Fengqing, Zhang Bing, Xu Zhiwen, Jiang Chaoyuan, Nei Mincai, Xu Lei, Zhao Jun, Deng Huidan, Sun Xiangang, Zhou Yuancheng, Zhu Ling
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
College of Animal Science, Xichang University, Xichang, China.
Front Vet Sci. 2022 Apr 25;9:883607. doi: 10.3389/fvets.2022.883607. eCollection 2022.
Getah virus (GETV) is a zoonotic arbovirus that can cause infection in many animals. It can cause pyrexia and reproductive losses in animals. The objective of the study was to explore the effects of GETV on male reproductive ability. Male mice were injected with 100 × TCID/0.1 ml in a volume of 100-μL GETV in their hindquarter muscle, resulting in decreased semen quality and testicular histopathological changes, and the virus was detected in the testes. At 0.5 dpi (day post-infection), male mice showed decreased sperm density, motility, and decreased serum testosterone concentration, an increased sperm malformation rate, vacuoles in spermatogonial cells/spermatocytes in spermatogenic tubules, and the highest virus copies in testis. At 2 dpi, the sperm density and motility reached the lowest value of 3.99 × 10/ml and 62.03%, and the malformation rate reached 43.67%. At 28 dpi, the sperm indexes of the experimental group gradually approached that of the control group, but there were still significant differences. Since then, histopathological changes have worsened, with the most severe histopathological changes at 7 dpi and gradual recovery. Up to 14 dpi, the virus was detected by qRT-PCR and immunohistochemistry, which showed that the virus was only present in the testicular interstitium. GETV infection can rapidly enter the testis of mice and reduce the semen quality of mice, which needs to be paid attention to in the prevention and control of GETV.
盖塔病毒（GETV）是一种人畜共患虫媒病毒，可在多种动物中引起感染。它可导致动物发热和生殖损失。本研究的目的是探讨GETV对雄性生殖能力的影响。将100×TCID/0.1 ml、体积为100 μL的GETV注射到雄性小鼠的后肢肌肉中，导致精液质量下降和睾丸组织病理学变化，并且在睾丸中检测到该病毒。在感染后0.5天（dpi），雄性小鼠精子密度、活力降低，血清睾酮浓度降低，精子畸形率增加，生精小管中的精原细胞/精母细胞出现空泡，睾丸中的病毒拷贝数最高。在2 dpi时，精子密度和活力达到最低值，分别为3.99×10/ml和62.03%，畸形率达到43.67%。在28 dpi时，实验组的精子指标逐渐接近对照组，但仍存在显著差异。此后，组织病理学变化加重，在7 dpi时组织病理学变化最严重，随后逐渐恢复。直至14 dpi，通过qRT-PCR和免疫组化检测到病毒，表明病毒仅存在于睾丸间质中。GETV感染可迅速进入小鼠睾丸并降低小鼠精液质量，这在GETV的防控中需要引起重视。
