Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
Laboratório de Microbiologia Molecular, Instituto de Ciências da Saúde, Universidade Feevale, Novo Hamburgo, Rio Grande do Sul, Brazil.
Infect Genet Evol. 2021 Aug;92:104862. doi: 10.1016/j.meegid.2021.104862. Epub 2021 Apr 10.
South American fur seals (Arctocephalus australis) are believed to reach the coast of Rio Grande do Sul (RS) through sea currents. They live in colonies and are frequently found resting on the beach. However, it is also common to find dead pinnipeds on beaches, sharing the environment with humans, domestic animals and other wild species on the coast and facilitating the transmission of pathogens. In the present study, a metagenomic approach was applied to evaluate the viral diversity in organs of fur seals found deceased along the coast of the state of RS, southern Brazil. The lungs and spleens of 29 animals were collected, macerated individually, pooled separately (one pool for lungs and another for spleens) and sequenced using the Illumina MiSeq platform. Sequences more closely related to members of the Anelloviridae and Circoviridae families were detected. Nine putative new species of anellovirus and one putative new genus, named Nitorquevirus, were described. Additionally, the circovirus sequences found in the lungs of A. australis have a common ancestor with PCV3, a proposed swine pathogen. Our study expanded the knowledge about viral communities in pinnipeds and could be useful for monitoring new viruses and potential viral sharing among wildlife, domestic animals, and humans.
南美海狗（Arctocephalus australis）据信是通过海流到达南里奥格兰德州（RS）的海岸。它们生活在群体中，经常在海滩上休息。然而，在海滩上发现死亡的鳍足类动物也很常见，它们与人类、家畜和沿海的其他野生动物共享环境，这促进了病原体的传播。在本研究中，采用宏基因组学方法评估了在巴西南部南里奥格兰德州海岸发现的死亡海狗的器官中的病毒多样性。收集了 29 只动物的肺和脾脏，单独进行粉碎，分别进行混合（一个肺混合池和一个脾混合池），并使用 Illumina MiSeq 平台进行测序。检测到与圆环病毒科和细小病毒科成员密切相关的序列。描述了 9 种新的拟神经病毒和一种新的属，命名为 Nitorquevirus。此外，在 A. australis 的肺中发现的圆环病毒序列与 PCV3 有共同的祖先，PCV3 是一种被提议的猪病原体。我们的研究扩展了对鳍足类动物病毒群落的认识，可用于监测野生动物、家畜和人类之间的新病毒和潜在的病毒共享。
