Crane Adele, Goebel Michael E, Kraberger Simona, Stone Anne C, Varsani Arvind
School of Life Sciences, Arizona State University, Tempe, AZ, 85287-5001, USA.
Antarctic Ecosystem Research Division, NOAA/NMFS/Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA, 92037-1508, USA.
Virus Genes. 2018 Oct;54(5):719-723. doi: 10.1007/s11262-018-1585-9. Epub 2018 Jul 3.
Viral diversity associated with Antarctic wildlife remains poorly studied. Nonetheless, over the past 5 years, there has been a concerted effort using viral metagenomics approaches to identify and characterize viruses associated with Antarctic pinniped and avian species. Here we used a viral metagenomics approach to identify circular DNA viruses in buccal swab samples from Antarctic fur seals (Arctocephalus gazella) breeding on Livingston Island, Antarctica during the 2016/2017 field season. We identified two new lineages of anelloviruses, torque teno Arctocephalus gazella virus (TTAgV)-1 (2083 nts) and -2 (2127-2143 nts), which are most closely related to and cluster with a previously identified anellovirus associated with California sea lions (Zalophus californianus) sharing ~ 60% genome-wide pairwise identity. The ORF1 of TTAgVs share 26-41% amino acid similarity to the ORF1 of other previously identified pinniped-associated anelloviruses.
与南极野生动物相关的病毒多样性仍未得到充分研究。尽管如此，在过去5年里，人们齐心协力采用病毒宏基因组学方法来识别和表征与南极鳍足类动物和鸟类相关的病毒。在此，我们采用病毒宏基因组学方法，在2016/2017野外考察季于南极利文斯顿岛繁殖的南极毛皮海狮（南极海狗）的口腔拭子样本中识别环状DNA病毒。我们识别出了两种新的环曲病毒谱系，即南极海狗环曲病毒（TTAgV）-1（2083个核苷酸）和-2（2127 - 2143个核苷酸），它们与之前识别出的一种与加利福尼亚海狮相关的环曲病毒关系最为密切且聚集在一起，全基因组两两比对相似度约为60%。TTAgV的ORF1与其他之前识别出的与鳍足类动物相关的环曲病毒的ORF1氨基酸相似度为26% - 41%。
