Chiappetta Catarina Marcon, Cibulski Samuel Paulo, Lima Francisco Esmaile Sales, Varela Ana Paula Muterle, Amorim Derek Blaese, Tavares Maurício, Roehe Paulo Michel
Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul (RS), 90050-170, Brazil.
Laboratório de Virologia Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Avenida Bento Gonçalves 9090, Porto Alegre, Rio Grande do Sul (RS), 9154-000, Brazil.
Ecohealth. 2017 Mar;14(1):69-77. doi: 10.1007/s10393-016-1195-8. Epub 2016 Nov 1.
In some regions, little is known about exposure to viruses in coastal marine mammals. The present study aimed to detect viral RNA or DNA in 23 free-ranging fur seals on the northern coastline of Rio Grande do Sul State, Brazil. Polymerase chain reaction was used to detect nucleic acids of circoviruses, adenoviruses, morbilliviruses, vesiviruses, and coronaviruses in the feces from twenty-one South American fur seals (Arctocephalus australis) and two Subantarctic fur seals (A. tropicalis). Adenovirus DNA fragments were detected in two South American fur seals; nucleotide sequences of these fragments revealed a high degree of similarity to human adenovirus type C. Circovirus DNA fragments were detected in six animals of the same species. Two were phylogenetically similar to the Circovirus genus, whereas the other four nucleotide fragments showed no similarity to any of the known genera within the family Circoviridae. RNA fragments indicating the presence of coronavirus, vesivirus, and morbillivirus were not detected. These findings suggest that adenoviruses and circoviruses are circulating in fur seal populations found along the coast of Rio Grande do Sul State, Brazil.
在一些地区，人们对沿海海洋哺乳动物接触病毒的情况知之甚少。本研究旨在检测巴西南里奥格兰德州北部海岸线23只自由放养海狗的病毒RNA或DNA。采用聚合酶链反应检测21只南美海狗（南极海狗）和2只亚南极海狗粪便中环状病毒、腺病毒、麻疹病毒、杯状病毒和冠状病毒的核酸。在两只南美海狗中检测到腺病毒DNA片段；这些片段的核苷酸序列显示与C型人类腺病毒高度相似。在同一物种的6只动物中检测到环状病毒DNA片段。其中2个在系统发育上与环状病毒属相似，而其他4个核苷酸片段与环状病毒科内任何已知属均无相似性。未检测到表明存在冠状病毒、杯状病毒和麻疹病毒的RNA片段。这些发现表明，腺病毒和环状病毒在巴西南里奥格兰德州沿海发现的海狗种群中传播。
