Judák Péter, Esposito Simone, Coppieters Gilles, Van Eenoo Peter, Deventer Koen
Department of Diagnostic Sciences, Doping Control Laboratory, Ghent University, Zwijnaarde, Belgium.
ADME/DMPK Department, Drug Discovery Division, IRBM S.p.A, Pomezia, Rome, Italy.
J Chromatogr B Analyt Technol Biomed Life Sci. 2021 Jan 15;1173:122551. doi: 10.1016/j.jchromb.2021.122551.
Small peptides are handled in the field of sports drug testing analysis as a separate group doping substances. It is a diverse group, which includes but is not limited to growth hormone releasing-factors and gonadotropin-releasing hormone analogues. Significant progress has been achieved during the past decade in the doping control analysis of these peptides. In this article, achievements in the application of liquid chromatography-mass spectrometry-based methodologies are reviewed. To meet the augmenting demands for analyzing an increasing number of samples for the presence of an increasing number of prohibited small peptides, testing methods have been drastically simplified, whilst their performance level remained constant. High-resolution mass spectrometers have been installed in routine laboratories and became the preferred detection technique. The discovery and implementation of metabolites/catabolites in testing methods led to extended detection windows of some peptides, thus, contributed to more efficient testing in the anti-doping community.
小肽在运动药物检测分析领域被作为一类单独的兴奋剂物质来处理。这是一个多样化的类别，包括但不限于生长激素释放因子和促性腺激素释放激素类似物。在过去十年中，这些肽的兴奋剂检测分析取得了重大进展。本文综述了基于液相色谱-质谱方法的应用成果。为了满足对越来越多的样品进行分析以检测越来越多违禁小肽的不断增长的需求，检测方法已大幅简化，而其性能水平保持不变。高分辨率质谱仪已安装在常规实验室中，并成为首选的检测技术。检测方法中代谢物/分解代谢物的发现和应用延长了某些肽的检测窗口，从而有助于反兴奋剂领域进行更有效的检测。
