Beijing Anti-Doping Laboratory, Beijing Sport University, Beijing, People's Republic of China.
School of Sport Science, Beijing Sport University, Beijing, People's Republic of China.
Anal Bioanal Chem. 2024 Nov;416(26):5655-5669. doi: 10.1007/s00216-024-05480-w. Epub 2024 Aug 24.
Dried blood spot (DBS) technique has become a new popular topic in anti-doping field in recent years due to its advantages of sample stability and easy operation. It can be employed as a supplementary method to routine urine analysis. However, the small volume of DBS samples (usually 10-20 μL) significantly reduces the application value of this technique. Therefore, the development of sensitive detection methods for the analysis of prohibited substances in DBS is particularly important. In this study, based on the characteristics of low molecular mass peptide (LMMP) drugs, systematic optimization strategies were utilized for the first time to establish a sensitive detection method for LMMPs in DBS. Without using DMSO to enhance mass spectrometry ionization efficiency of peptides, the limits of detection (LOD) ranged between 0.05 and 3.74 ng/mL, significantly better than the previously reported method (0.5-20 ng/mL). This method was validated according to the guidelines of the World Anti-Doping Agency (WADA), and corresponding post-administration study was conducted, demonstrating that the method could be applied to routine analysis of LMMP drugs in DBS. Moreover, since DMSO is not involved, this method also has the potential to simultaneously detect both LMMP and small molecular drugs.
干血斑（DBS）技术由于其样本稳定性和操作简便的优点，近年来已成为反兴奋剂领域的一个新热点。它可以作为常规尿液分析的补充方法。然而，DBS 样本的体积较小（通常为 10-20 μL），这极大地降低了该技术的应用价值。因此，开发用于分析 DBS 中禁用物质的灵敏检测方法尤为重要。在本研究中，基于低分子质量肽（LMMP）药物的特点，我们首次利用系统的优化策略，建立了一种用于 DBS 中 LMMP 的灵敏检测方法。该方法无需使用 DMSO 增强肽的质谱离子化效率，检测限（LOD）范围在 0.05 至 3.74ng/mL 之间，明显优于先前报道的方法（0.5-20ng/mL）。该方法按照世界反兴奋剂机构（WADA）的指南进行了验证，并进行了相应的给药后研究，表明该方法可用于 DBS 中 LMMP 药物的常规分析。此外，由于不涉及 DMSO，该方法还有望同时检测 LMMP 和小分子药物。
