用等电聚焦法纯化葡萄球菌肠毒素D
Chromatofocusing in the purification of staphylococcal enterotoxin D.
作者信息
Lei Z, Reiser R F, Bergdoll M S
机构信息
Food Research Institute, University of Wisconsin, Madison 53706.
出版信息
J Clin Microbiol. 1988 Jun;26(6):1236-7. doi: 10.1128/jcm.26.6.1236-1237.1988.
Abstract
A chromatofocusing procedure for the purification of staphylococcal enterotoxin D was developed. The purification included the removal of the toxic protein from culture supernatant fluids of Staphylococcus aureus 1151m by batch adsorption with CG-50 resin, chromatofocusing on Polybuffer Exchanger 94, and gel permeation chromatography on Sephacryl S-200. The purity of the staphylococcal enterotoxin D obtained was approximately 98%.
摘要
开发了一种用于纯化葡萄球菌肠毒素D的色谱聚焦方法。纯化过程包括通过用CG - 50树脂分批吸附从金黄色葡萄球菌1151m的培养上清液中去除有毒蛋白质，在聚缓冲液交换剂94上进行色谱聚焦，以及在Sephacryl S - 200上进行凝胶渗透色谱。所获得的葡萄球菌肠毒素D的纯度约为98%。
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