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Production of enterotoxin a.肠毒素A的产生
Appl Microbiol. 1966 Nov;14(6):966-72. doi: 10.1128/am.14.6.966-972.1966.
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Chromatofocusing: a new method for purification of staphylococcal enterotoxins B and C1.层析聚焦法：一种纯化葡萄球菌肠毒素B和C1的新方法。
Appl Environ Microbiol. 1983 Dec;46(6):1323-30. doi: 10.1128/aem.46.6.1323-1330.1983.
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Purification and some physicochemical properties of toxic-shock toxin.中毒性休克毒素的纯化及某些物理化学性质
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4
Simple production of staphylococcal enterotoxins (SET) with chromatofocusing and isoelectrofocusing in flat bed gels.利用平板凝胶中的色谱聚焦和等电聚焦简单生产葡萄球菌肠毒素（SET）。
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Identification, purification, and some physicochemical properties of staphylococcal enterotoxin C3.葡萄球菌肠毒素C3的鉴定、纯化及某些理化性质
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Detecting the enterotoxigenicity of Staphylococcus aureus strains.检测金黄色葡萄球菌菌株的肠毒素产生能力。
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Purification and some physicochemical properties of staphylococcal enterotoxin D.
Biochemistry. 1979 May 15;18(10):1937-42. doi: 10.1021/bi00577a013.

用等电聚焦法纯化葡萄球菌肠毒素D

Chromatofocusing in the purification of staphylococcal enterotoxin D.

作者信息

Lei Z, Reiser R F, Bergdoll M S

机构信息

Food Research Institute, University of Wisconsin, Madison 53706.

出版信息

J Clin Microbiol. 1988 Jun;26(6):1236-7. doi: 10.1128/jcm.26.6.1236-1237.1988.

DOI:10.1128/jcm.26.6.1236-1237.1988
PMID:3384936
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC266573/
Abstract

A chromatofocusing procedure for the purification of staphylococcal enterotoxin D was developed. The purification included the removal of the toxic protein from culture supernatant fluids of Staphylococcus aureus 1151m by batch adsorption with CG-50 resin, chromatofocusing on Polybuffer Exchanger 94, and gel permeation chromatography on Sephacryl S-200. The purity of the staphylococcal enterotoxin D obtained was approximately 98%.

摘要

开发了一种用于纯化葡萄球菌肠毒素D的色谱聚焦方法。纯化过程包括通过用CG - 50树脂分批吸附从金黄色葡萄球菌1151m的培养上清液中去除有毒蛋白质，在聚缓冲液交换剂94上进行色谱聚焦，以及在Sephacryl S - 200上进行凝胶渗透色谱。所获得的葡萄球菌肠毒素D的纯度约为98%。