Dada Felix Abayomi, Oyeleye Sunday Idowu, Adefegha Stephen Adeniyi, Babatola Leye Jonathan, Adebayo Adeniyi
Science Laboratory Technology Department (Biochemistry Unit), Federal Polytechnic Ede, Ede, Osun State, Nigeria.
Functional Foods and Nutraceuticals Research Laboratory, Biochemistry Department, Federal University of Technology, Akure, Nigeria.
J Complement Integr Med. 2021 Apr 13;18(4):727-735. doi: 10.1515/jcim-2020-0193.
This study was designed to evaluate the ameliorative effect of almond () leaf (ALE) and stem bark (ABE) extracts on the enzyme activities and oxidative stress markers in the brain and liver tissues of cyclosporine-A (CsA) stressed male albino rats.
Eighty-eight adult male rats weighing between 200 and 220 g were randomly distributed to into 11 groups (n=8) and different doses (100 and 200 mg/kg bwt.) of ALE and ABE were administered through oral gavages to the normal rats and 50 mg/kg/bwt/day CsA-stressed, while normal control rats was given a saline solution (p.o), and the treatment lasted for 14 days. Blood plasma, liver and brain tissues were prepared for biochemical analysis.
Neuronal [acetylcholinesterase (AChE) and butrylcholinesterase (BChE) and arginase] enzyme activities and thiobarbituric acid reactive species (TBARS) level, plasma aspartate transferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities, liver non-protein thiol (NPSH) level were analyzed. The results revealed that, the administration of CsA induced a significant increase in neuronal AChE, BChE, arginase, TBARS level, but decreased nitric oxide (NO) level. CsA also increased ALT, AST, and ALP activities in the blood plasma of CsA stress rats compared to normal control, but were significantly reversed respectively (p<0.001) upon treatment with the ALE and ABE dose-dependently.
The study revealed that ALE and ABE could prevent neuronal dysfunction and liver toxicity induced by CsA administration, however, higher dose (200 mg/kg) of the studied extracts appears to be more potent.
本研究旨在评估杏仁叶提取物（ALE）和茎皮提取物（ABE）对环孢素A（CsA）应激雄性白化大鼠脑和肝组织中酶活性及氧化应激标志物的改善作用。
将88只体重在200至220克之间的成年雄性大鼠随机分为11组（每组n = 8），分别通过灌胃给予正常大鼠不同剂量（100和200毫克/千克体重）的ALE和ABE，以及给予50毫克/千克体重/天CsA应激的大鼠，正常对照大鼠给予生理盐水（口服），治疗持续14天。制备血浆、肝脏和脑组织用于生化分析。
分析了神经元[乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）和精氨酸酶]的酶活性、硫代巴比妥酸反应性物质（TBARS）水平、血浆天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和碱性磷酸酶（ALP）活性以及肝脏非蛋白硫醇（NPSH）水平。结果显示，给予CsA可导致神经元AChE、BChE、精氨酸酶、TBARS水平显著升高，但一氧化氮（NO）水平降低。与正常对照相比，CsA还增加了CsA应激大鼠血浆中的ALT、AST和ALP活性，但经ALE和ABE剂量依赖性治疗后分别显著逆转（p < 0.001）。
该研究表明，ALE和ABE可预防CsA给药诱导的神经元功能障碍和肝脏毒性，然而，所研究提取物的较高剂量（200毫克/千克）似乎更有效。
