School of Pharmaceutical sciences, Qilu University of Technology (Shandong Academy of Sciences), 19th Keyuan Road, Jinan, Shandong 250014, People's Republic of China.
Department of Chemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, People's Republic of China.
Anal Chem. 2021 Apr 27;93(16):6342-6347. doi: 10.1021/acs.analchem.1c00260. Epub 2021 Apr 14.
The size and distribution of matrix crystals deposited on the surface of a tissue section play a key role in the performance of MALDI mass spectrometry imaging (MALDI-MSI). In this study, uniform distribution and a restricted size of matrix crystals were achieved via a homemade matrix sublimation device with controllable crystallization temperature. The crystallization temperature was stably controlled at a subzero temperature, and homogeneous matrix crystals with diameters <0.2 μm were generated on the sample surface. Typical MALDI-MSI experiments of endogenous and exogenous components in the tissues of strawberries, kidneys, and mussels were conducted to examine the performance of the sublimator. Good reproducibility of MALDI-MSI was achieved, and the quality of ion images was significantly improved. These results demonstrate that the developed sublimator should have potential in matrix deposition for further high resolution MALDI-MSI application.
基质晶体在组织切片表面的大小和分布对 MALDI 质谱成像（MALDI-MSI）的性能起着关键作用。在这项研究中，通过自制的具有可控结晶温度的基质升华装置实现了基质晶体的均匀分布和受限尺寸。结晶温度稳定控制在零下温度，在样品表面生成了直径<0.2μm 的均匀基质晶体。对草莓、肾脏和贻贝组织中的内源性和外源性成分进行了典型的 MALDI-MSI 实验，以检验升华器的性能。MALDI-MSI 具有良好的重现性，离子图像的质量得到了显著改善。这些结果表明，所开发的升华器在进一步的高分辨率 MALDI-MSI 应用中具有基质沉积的潜力。
