Department of Physical Chemistry, Faculty of Science and Technology , University of the Basque Country (UPV/EHU) , Barrio Sarriena S/N, Leioa 48940 , Spain.
Anal Chem. 2019 Jan 2;91(1):803-807. doi: 10.1021/acs.analchem.8b04765. Epub 2018 Dec 18.
Sublimation is a widely used method for matrix deposition in imaging mass spectrometry experiments. Still, most of the time, standard glass sublimators are used for this purpose, which do not enable optimal matrix deposition reproducibility, compromising inter-experiment comparison of the results. Here, we present an in-house designed stainless steel sublimator in which the parameters that have the strongest influence over matrix deposition reproducibility can be easily monitored. Using sections of human colon biopsies, we demonstrate the capabilities of this new prototype.
升华是一种广泛应用于成像质谱实验中基质沉积的方法。然而，大多数情况下，标准的玻璃升华器用于此目的，这不能实现最佳的基质沉积重现性，从而影响实验结果的可比较性。在这里，我们展示了一种内部设计的不锈钢升华器，其中可以轻松监测对基质沉积重现性有最强影响的参数。我们使用人类结肠活检切片来演示这个新原型的能力。
