Shanmugaraj Nandhakumar, Rutten Twan, Svatoš Aleš, Schnurbusch Thorsten, Mock Hans-Peter
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, OT Gatersleben, 06466, Seeland, Germany.
Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, 07745, Jena, Germany.
J Am Soc Mass Spectrom. 2023 Mar 1;34(3):513-517. doi: 10.1021/jasms.2c00301. Epub 2023 Feb 3.
Sublimation is one of the preferred methods of choice for matrix deposition in high spatial resolution MALDI mass spectrometry imaging (MALDI-MSI) experiments. However, reproducibility and time are the major concerns for this setup. Here we present a lab-made glass sublimator with significant improvements in fine control of the vacuum with real-time monitoring and a rapid sublimation process of only 22 min. This method yielded reproducible homogeneous matrix crystals of <1 μm on the sample surface. MALDI-MSI was performed in tissue sections of barley inflorescence meristems at 15 μm spatial resolution, thus demonstrating its efficiency. Overall, we believe these simple yet effective new modifications can be easily adapted to the standard glass sublimation devices to achieve highly reproducible matrix deposition for high spatial resolution MALDI-MSI.
升华是高空间分辨率基质辅助激光解吸电离质谱成像（MALDI-MSI）实验中基质沉积的首选方法之一。然而，该装置的可重复性和时间是主要问题。在此，我们展示了一种实验室自制的玻璃升华器，它在真空精细控制方面有显著改进，具备实时监测功能，且升华过程迅速，仅需22分钟。该方法在样品表面产生了尺寸小于1μm的可重复均匀基质晶体。以15μm的空间分辨率在大麦花序分生组织的组织切片上进行了MALDI-MSI，从而证明了其有效性。总体而言，我们认为这些简单而有效的新改进可以轻松应用于标准玻璃升华装置，以实现用于高空间分辨率MALDI-MSI的高度可重复基质沉积。
