Izutsu T, Kagabu T, Nishiya I, Wied G L
Department of Obstetrics and Gynecology, Iwate Medical University.
Nihon Sanka Fujinka Gakkai Zasshi. 1988 May;40(5):621-6.
The DNA content of the nuclei of cancer cells of 12 cases of cervical cancer and 2 cases of vaginal cancer, treated with radiotherapy, were studied in 50 specimens. Specimens were taken from each case before radiotherapy and at the totals of 1,000 rad, 2,000 rad, 3,000 rad and 4,500 rad (or 5,000 rad). All specimens were stained by the Papanicolaou method and were analyzed by rapid high-resolution cytometry. Total optical density, mean nuclear area and the 5N-exceeding rate (5NER) increased gradually following irradiation. Cancer cells disappeared in good response cases before 3,000 rad. Eight smears with a 5NER under 100 at the dose of 3,000 rad or more seemed to be poor response cases. Low 5NER and low mean nuclear areas were observed in both patients who died with persistent disease after radiotherapy, as well as in one case treated with chemotherapy for persistent disease after radiotherapy.
对12例宫颈癌和2例阴道癌患者经放疗后的50份标本进行了癌细胞核DNA含量研究。标本取自每个病例放疗前以及总剂量达1000拉德、2000拉德、3000拉德和4500拉德（或5000拉德）时。所有标本均采用巴氏染色法染色，并通过快速高分辨率细胞术进行分析。照射后，总光密度、平均核面积和5倍体超标率（5NER）逐渐增加。在3000拉德之前，反应良好的病例中癌细胞消失。在剂量达3000拉德或更高时，5NER低于100的8份涂片似乎是反应不佳的病例。放疗后死于持续性疾病的患者以及1例放疗后因持续性疾病接受化疗的患者均观察到低5NER和低平均核面积。
