Bélanger F, Berthiaume L, Alain R, Lussier G, Trudel M
Centre de Recherche en Virologie, Institut Armand-Frappier, Université du Québec, Laval, Canada.
J Gen Virol. 1988 Jun;69 ( Pt 6):1421-4. doi: 10.1099/0022-1317-69-6-1421.
Electron microscopic examination of ultrathin sections of a continuous cell line of ovine kidney (OK) origin, infected by bovine respiratory syncytial virus (BRSV), revealed the presence of well defined bridges between virus particles. This is the first report of this novel structure. Observation of ultrathin sections of human RSV Long strain also grown on OK cells did not show inter-particle bridges and therefore suggested that this structure could be specific to BRSV. The biological significance of these bridges is not clear at this time; a possibility is that the bridges are formed by the fusion protein of BRSV which is known to cause cell fusion. Besides the structural implications, the importance is in relation to purification strategies for this virus, which must now take into account that most of the viral particles occur in large aggregates.
对源自绵羊肾脏（OK）的连续细胞系超薄切片进行电子显微镜检查，该细胞系感染了牛呼吸道合胞病毒（BRSV），结果显示病毒颗粒之间存在界限分明的桥状物。这是关于这种新结构的首次报道。对同样在OK细胞上培养的人呼吸道合胞病毒长株超薄切片的观察未发现颗粒间桥状物，因此表明这种结构可能是BRSV特有的。目前这些桥状物的生物学意义尚不清楚；一种可能性是这些桥状物由已知会导致细胞融合的BRSV融合蛋白形成。除了结构方面的影响，其重要性还与该病毒的纯化策略有关，现在必须考虑到大多数病毒颗粒以大聚集体形式存在。
