Masot A J, Gómez-Tejedor C, Tovar I, Gázquez A, Redondo E
Fac. Vet. Med., Cáceres, Spain.
Eur J Histochem. 1993;37(1):75-82.
The experiments were designed to analyse the efficiency of the Indirect Fluorescent Antibody Test (IFAT), the Avidin-Biotin-Peroxidase Complex (ABPC) and Transmission Electron Microscopy (TEM) in detecting Bovine Respiratory Syncytial Virus (BRSV) antigen in ovine lung. 25 gnotobiotic Merino lambs each received an intratracheal injection of 20 ml of BRSV infected cell culture suspension (strain NMK-7) diluted at the concentration of 1.26 x 10(6) TCID50/ml. Results showed that the immunological techniques were more efficient than transmission electron microscopy in detecting BRSV antigen. IFAT and ABPC yielded identical results. However, difficulties in interpreting aspecific fluorescence, coupled with the ease of identification of pneumonic areas from paraffin-embedded tissues, suggest that ABPC is the most suitable technique for identifying BRSV antigen in the lungs of experimentally-infected sheep.
这些实验旨在分析间接荧光抗体试验（IFAT）、抗生物素蛋白-生物素-过氧化物酶复合物（ABPC）和透射电子显微镜（TEM）检测绵羊肺中牛呼吸道合胞病毒（BRSV）抗原的效率。25只无菌美利奴羔羊每只经气管内注射20毫升以1.26×10(6) 半数组织培养感染剂量（TCID50）/毫升浓度稀释的BRSV感染细胞培养悬液（NMK-7株）。结果表明，在检测BRSV抗原方面，免疫技术比透射电子显微镜更有效。IFAT和ABPC产生了相同的结果。然而，由于在解释非特异性荧光方面存在困难，再加上从石蜡包埋组织中易于识别肺炎区域，这表明ABPC是鉴定实验感染绵羊肺中BRSV抗原的最合适技术。
