Zhang Dawei, Du Yunyan, He Dan, Zhou Dinggang, Wu Jinfeng, Peng Jiashi, Liu Lili, Liu Zhongsong, Yan Mingli
School of Life Science, Hunan University of Science and Technology, Xiangtan, China.
Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, Xiangtan, China.
Front Genet. 2021 Mar 29;12:655885. doi: 10.3389/fgene.2021.655885. eCollection 2021.
The contamination of soils with cadmium (Cd) has become a serious environmental issue that needs to be addressed. Elucidating the mechanisms underlying Cd accumulation may facilitate the development of plants that accumulate both high and low amounts of Cd. In this study, a combination of phenotypic, physiological, and comparative transcriptomic analyses was performed to investigate the effects of different Cd concentrations (0, 5, 10, 30, 50 mg/kg) on L. Our results suggest that L. seedlings had a degree of tolerance to the 5 mg/kg Cd treatment, whereas higher Cd stress (10-50 mg/kg) could suppress the growth of L. seedlings. The contents of soluble protein, as well as MDA (malondialdehyde), were increased, but the activities of CAT (catalase) enzymes and the contents of soluble sugar and chlorophyll were decreased, when L. was under 30 and 50 mg/kg Cd treatment. Comparative transcriptomic analysis indicated that , and were down-regulated, but and were up-regulated, which might contribute to cell wall integrity maintenance. Moreover, the down-regulation of and up-regulation of , , and might also play roles in reducing Cd toxicity in roots. Taken together, the results of our study may help to elucidate the mechanisms underlying the response of L. to various concentrations of Cd.
土壤镉（Cd）污染已成为一个亟待解决的严重环境问题。阐明Cd积累的潜在机制可能有助于培育出Cd积累量高或低的植物。在本研究中，结合表型、生理和比较转录组分析，研究了不同Cd浓度（0、5、10、30、50 mg/kg）对[植物名称未给出]的影响。我们的结果表明，[植物名称未给出]幼苗对5 mg/kg Cd处理具有一定的耐受性，而较高的Cd胁迫（10 - 50 mg/kg）会抑制[植物名称未给出]幼苗的生长。当[植物名称未给出]处于30和50 mg/kg Cd处理时，可溶性蛋白以及丙二醛（MDA）的含量增加，但过氧化氢酶（CAT）的活性以及可溶性糖和叶绿素的含量降低。比较转录组分析表明，[基因名称未给出]下调，但[基因名称未给出]上调，这可能有助于维持细胞壁完整性。此外，[基因名称未给出]的下调以及[基因名称未给出]、[基因名称未给出]和[基因名称未给出]的上调也可能在降低根部Cd毒性方面发挥作用。综上所述，我们的研究结果可能有助于阐明[植物名称未给出]对不同浓度Cd响应的潜在机制。
