Zhang Zhong-Wei, Dang Tao-Tao, Yang Xin-Yue, Xie Lin-Bei, Chen Yang-Er, Yuan Ming, Chen Guang-Deng, Zeng Jian, Yuan Shu
College of Resources, Sichuan Agricultural University, Chengdu 611130, China.
College of Life Science, Sichuan Agricultural University, Ya'an 625014, China.
Int J Mol Sci. 2024 Dec 27;26(1):129. doi: 10.3390/ijms26010129.
Previous studies have demonstrated that γ-Aminobutyric acid (GABA) effectively alleviates heavy metal stresses by maintaining the redox balance and reducing the accumulation of reactive oxygen species (ROS). However, little is known about the role of GABA on programmed cell death (PCD) under Cd treatments in plants. The present study investigated the effects of GABA on Cd-induced PCD in two species, oilseed rape (, ), and black mustard (, ). We observed that GABA significantly alleviated Cd-induced PCD by enhancing antioxidant systems, inhibiting chromatin condensation in the nucleus, and reducing DNA fragmentation under Cd stress. Moreover, GABA may not only reduce caspase-3-like activity by repressing gene expression, but also regulate transcription of PCD-related genes. showed lower Cd accumulation and lower tolerance, with more pronounced PCD, compared with . Our results provide new insights into the mechanism that GABA enhances Cd tolerance in plants.
先前的研究表明，γ-氨基丁酸（GABA）通过维持氧化还原平衡和减少活性氧（ROS）的积累，有效缓解重金属胁迫。然而，关于GABA在镉处理下对植物程序性细胞死亡（PCD）的作用知之甚少。本研究调查了GABA对两种植物，即油菜（Brassica napus）和黑芥（Brassica nigra）中镉诱导的PCD的影响。我们观察到，GABA通过增强抗氧化系统、抑制细胞核中的染色质凝聚以及减少镉胁迫下的DNA片段化，显著缓解了镉诱导的PCD。此外，GABA不仅可以通过抑制基因表达降低类半胱天冬酶-3活性，还可以调节PCD相关基因的转录。与黑芥相比，油菜表现出较低的镉积累和耐受性，PCD更明显。我们的研究结果为GABA增强植物对镉耐受性的机制提供了新的见解。
