Owagboriaye Folarin, Mesnage Robin, Dedeke Gabriel, Adegboyega Taofeek, Aladesida Adeyinka, Adeleke Mistura, Owa Stephen, Antoniou Michael N
Department of Zoology and Environmental Biology, Olabisi Onabanjo University, Ago-Iwoye, Nigeria.
Department of Medical and Molecular Genetics, Kings College London, United Kingdom.
Toxicol Rep. 2021 Mar 31;8:753-758. doi: 10.1016/j.toxrep.2021.03.021. eCollection 2021.
While the impact of glyphosate-based herbicides (GBHs) on earthworms has been studied, little is known about their effects on the earthworm gut microbiome. This study investigated the impact of a GBH on the gut microbial communities of three earthworm species ( and ). Earthworm species accommodated in soil were sprayed with 115.49 mL/m² of Roundup® Alphée or water. Gut microbiome composition was analysed using 16S rRNA Bacterial Tag-Encoded FLX Amplicon Pyrosequencing (bTEFAP) at the 8th week post-herbicide application. A profound shift in bacterial populationswas observed in all exposed earthworms with becoming the dominant phylum. Affected bacteria were mostly from the genus , and , which together represented approximately 80 % of the total abundance assigned at the genus level in exposed earthworms, while they were present at a minor abundance (∼1%) in unexposed earthworms. Although consistent results were observed between the three groups of worm species, it is not possible to generalize these outcomes due to a lack of biological replicates, which does not allow for inferential statistical analysis. Nevertheless, our study is the first to report the effects of a GBH on the earthworm gut microbiome and paves the way for future more comprehensive investigations.
虽然基于草甘膦的除草剂（GBHs）对蚯蚓的影响已得到研究，但对其对蚯蚓肠道微生物组的影响却知之甚少。本研究调查了一种GBH对三种蚯蚓（ 和 ）肠道微生物群落的影响。将饲养在土壤中的蚯蚓用115.49 mL/m²的农达®Alphée或水进行喷洒。在除草剂施用后第8周，使用16S rRNA细菌标签编码FLX扩增子焦磷酸测序（bTEFAP）分析肠道微生物组组成。在所有暴露的蚯蚓中均观察到细菌种群发生了显著变化， 成为优势门类。受影响的细菌大多来自 属、 属和 属，在暴露蚯蚓中，这三个属的细菌在属水平上的总丰度约占80%，而在未暴露的蚯蚓中，它们的丰度较低（约1%）。尽管在三组蚯蚓物种之间观察到了一致的结果，但由于缺乏生物学重复样本，无法对这些结果进行归纳总结，也就无法进行推断性统计分析。然而，我们的研究首次报告了GBH对蚯蚓肠道微生物组的影响，为未来更全面的研究铺平了道路。
