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以水为溶剂测定大米的总抗氧化能力和总酚含量

The Total Antioxidant Capacity and the Total Phenolic Content of Rice Using Water as a Solvent.

作者信息

Priyanthi C, Sivakanesan R

机构信息

Faculty of Technology, University of Jaffna, Sri Lanka.

Faculty of Medicine, University of Peradeniya, Sri Lanka.

出版信息

Int J Food Sci. 2021 Mar 27;2021:5268584. doi: 10.1155/2021/5268584. eCollection 2021.

Abstract

BACKGROUND

The present study evaluates the antioxidant properties of some Sri Lankan red rice varieties using water extracts.

METHODS

Water extracts of rice varieties Attakkari, Bg2907, and Bg407 were used in this study. The total antioxidant capacity was measured by ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and reducing power assays. The total phenolic content (TPC), total flavonoid content (TFC), monomeric anthocyanin, and condensed tannin contents were measured by Folin-Ciocalteu, aluminium chloride, pH differential, and vanillin assays, respectively.

RESULTS

It was observed that mean FRAP, DPPH, reducing power, TPC, TFC, monomeric anthocyanin content, and condensed tannin content were in the range of 0.561 ± 0.113 to 0.695 ± 0.077 mmol/100 g fresh weight (FW), 26.07 ± 3.08 to 53.66 ± 7.61 mg/mL FW, 33.49 ± 4.105.14 to 40.81 ± 3.65 mg/mL, 0.676 ± 0.078 to 0.900 ± 0.057 mg tannic acid equivalent (TAE)/g, 5.36 ± 0.75 to 6.38 ± 0.82 mg TAE/g FW, 0.0202 ± 0.005 to 0.0292 ± 0.009 mg/g FW, and 0.078 ± 0.015 to 0.104 ± 0.017 mg TAE/g FW, respectively. Significant differences were observed in DPPH, reducing power, and TPC among rice varieties ( < 0.05). Rice variety Attakkari had the highest total antioxidant capacity (TAC), scavenging activity, reducing power, TPC, TFC, monomeric anthocyanin content, and condensed tannin content followed by Bg2907 and Bg406.

CONCLUSION

Total phenolic compounds, total flavonoid, and condensed tannin are the major antioxidants in all three varieties of rice while the monomeric anthocyanin is only a minor antioxidant.

摘要

背景

本研究使用水提取物评估了一些斯里兰卡红米品种的抗氧化特性。

方法

本研究采用了Attakkari、Bg2907和Bg407水稻品种的水提取物。通过铁还原抗氧化能力(FRAP)、2,2-二苯基-1-苦基肼(DPPH)自由基清除和还原能力测定来测量总抗氧化能力。分别通过福林-酚试剂法、氯化铝法、pH差值法和香草醛法测定总酚含量(TPC)、总黄酮含量(TFC)、单体花青素和缩合单宁含量。

结果

观察到平均FRAP、DPPH、还原能力、TPC、TFC、单体花青素含量和缩合单宁含量分别在0.561±0.113至0.695±0.077毫摩尔/100克鲜重(FW)、26.07±3.08至53.66±7.61毫克/毫升FW、33.49±4.10至5.14至40.81±3.65毫克/毫升、0.676±0.078至0.900±0.057毫克单宁酸当量(TAE)/克、5.36±0.75至6.38±0.82毫克TAE/克FW、0.0202±0.005至0.0292±0.009毫克/克FW和0.078±0.015至0.104±0.017毫克TAE/克FW范围内。在水稻品种之间,DPPH、还原能力和TPC存在显著差异(<0.05)。Attakkari水稻品种具有最高的总抗氧化能力(TAC)、清除活性、还原能力、TPC、TFC、单体花青素含量和缩合单宁含量,其次是Bg2907和Bg406。

结论

总酚类化合物、总黄酮和缩合单宁是所有三个水稻品种中的主要抗氧化剂,而单体花青素只是一种次要的抗氧化剂。

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