Department of Physics, University of Bath, Bath, UK.
Translational Healthcare Technologies Team, Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, UK.
J Biophotonics. 2021 Oct;14(10):e202000488. doi: 10.1002/jbio.202000488. Epub 2021 Jul 4.
Using the shifted-excitation Raman difference spectroscopy technique and an optical fibre featuring a negative curvature excitation core and a coaxial ring of high numerical aperture collection cores, we have developed a portable, background and fluorescence free, endoscopic Raman probe. The probe consists of a single fibre with a diameter of less than 0.25 mm packaged in a sub-millimetre tubing, making it compatible with standard bronchoscopes. The Raman excitation light in the fibre is guided in air and therefore interacts little with silica, enabling an almost background free transmission of the excitation light. In addition, we used the shifted-excitation Raman difference spectroscopy technique and a tunable 785 nm laser to separate the fluorescence and the Raman spectrum from highly fluorescent samples, demonstrating the suitability of the probe for biomedical applications. Using this probe we also acquired fluorescence free human lung tissue data.
我们使用移频激发拉曼差谱技术和一根具有负曲率激发核以及同轴高数值孔径收集核环的光纤，开发了一种便携式、无背景和无荧光的内窥镜拉曼探头。该探头由一根直径小于 0.25mm 的光纤组成，封装在亚毫米级的管道中，与标准支气管镜兼容。光纤中的拉曼激发光在空气中传播，因此与二氧化硅的相互作用很小，从而实现了激发光的几乎无背景传输。此外，我们还使用移频激发拉曼差谱技术和可调谐 785nm 激光，从高荧光样品中分离出荧光和拉曼光谱，证明了该探头适用于生物医学应用。我们还使用该探头获取了无荧光的人体肺组织数据。
