Wood Harry Alexander Charles, Ehrlich Katjana, Yerolatsitis Stephanos, Kufcsák András, Quinn Tom Michael, Fernandes Susan, Norberg Dominic, Jenkins Nia Caitlin, Young Vikki, Young Irene, Hamilton Katie, Seth Sohan, Akram Ahsan, Thomson Robert Rodrick, Finlayson Keith, Dhaliwal Kevin, Stone James Morgan
Centre for Photonics and Photonic Materials, University of Bath, Bath, UK.
Translational Healthcare Technologies Group, Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, UK.
J Biophotonics. 2023 Feb;16(2):e202200141. doi: 10.1002/jbio.202200141. Epub 2022 Sep 21.
We present an endoscopic probe that combines three distinct optical fibre technologies including: A high-resolution imaging fibre for optical endomicroscopy, a multimode fibre for time-resolved fluorescence spectroscopy, and a hollow-core fibre with multimode signal collection cores for Raman spectroscopy. The three fibers are all enclosed within a 1.2 mm diameter clinical grade catheter with a 1.4 mm end cap. To demonstrate the probe's flexibility we provide data acquired with it in loops of radii down to 2 cm. We then use the probe in an anatomically accurate model of adult human airways, showing that it can be navigated to any part of the distal lung using a commercial bronchoscope. Finally, we present data acquired from fresh ex vivo human lung tissue. Our experiments show that this minimally invasive probe can deliver real-time optical biopsies from within the distal lung - simultaneously acquiring co-located high-resolution endomicroscopy and biochemical spectra.
我们展示了一种内窥镜探头，它结合了三种不同的光纤技术，包括：用于光学内镜显微镜检查的高分辨率成像光纤、用于时间分辨荧光光谱学的多模光纤，以及带有用于拉曼光谱学的多模信号收集芯的空心光纤。这三根光纤均封装在一根直径为1.2毫米、带有1.4毫米端帽的临床级导管内。为了证明该探头的灵活性，我们提供了在半径低至2厘米的环中使用它获取的数据。然后，我们在成人人类气道的解剖学精确模型中使用该探头，表明可以使用商用支气管镜将其导航到远端肺部的任何部位。最后，我们展示了从新鲜离体人类肺组织获取的数据。我们的实验表明，这种微创探头可以从远端肺部进行实时光学活检——同时获取共定位的高分辨率内镜显微镜图像和生化光谱。
