University of Bordeaux, CNRS, Bordeaux INP, LCPO, UMR 5629, 33600, Pessac, France.
Angew Chem Int Ed Engl. 2021 Jun 25;60(27):15036-15040. doi: 10.1002/anie.202102807. Epub 2021 May 26.
Biomimetic design to afford smart functional biomaterials with exquisite properties represents synthetic challenges and provides unique perspectives. In this context, elastin-like polypeptides (ELPs) recently became highly attractive building blocks in the development of lipoprotein-based membranes. In addition to the bioengineered post-translational modifications of genetically encoded recombinant ELPs developed so far, we report here a simple and versatile method to design biohybrid brush-like lipid-grafted-ELPs using chemical post-modification reactions. We have explored a combination of methionine alkylation and click chemistry to create a new class of hybrid lipoprotein mimics. Our design allowed the formation of biomimetic vesicles with controlled permeability, correlated to the temperature-responsiveness of ELPs.
仿生设计为具有精致特性的智能功能生物材料提供了合成挑战，并提供了独特的视角。在这种情况下，弹性蛋白样多肽（ELPs）最近成为脂蛋白基膜开发中极具吸引力的构建块。除了迄今为止通过基因工程修饰的重组 ELP 的生物工程后修饰外，我们在这里还报告了一种使用化学后修饰反应设计生物杂交刷状脂质接枝-ELP 的简单而通用的方法。我们探索了蛋氨酸烷基化和点击化学的组合，以创建一类新型的混合脂蛋白模拟物。我们的设计允许形成具有可控渗透性的仿生囊泡，与 ELPs 的温度响应性相关。
