Shanghai Burn Institute, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Shanghai 9th people's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
J Wound Care. 2021 Apr 1;30(Sup4):S14-S23. doi: 10.12968/jowc.2021.30.Sup4.S14.
This study aimed to investigate how adipose tissue-derived stem cells (ASCs) from diabetic and from non-diabetic rats affect wound healing in different microenvironments.
The two types of ASC-rich cells were distinguished by characteristic surface antigen detection. The ASC-rich cells were transplanted into the wounds of diabetic and non-diabetic rats. Wound healing rates were compared and the healing process in the wound margin sections was used to determine how ASC-rich cells affect wound healing in different microenvironments.
ASC density was decreased in diabetic rats. The generation time of ASC-rich cells from diabetic rats (d-ASC-rich cells) was longer than that of ASC-rich cells from non-diabetic rats. The number of pre-apoptotic cells in the third generation (passage 3) of d-ASC-rich cells was higher than that among the ASC-rich cells from non-diabetic rats. CD31 and CD34 expression was higher in d-ASC-rich cells than in ASC-rich cells from non-diabetic rats, whereas CD44 and CD105 expression was lower than that in ASC-rich cells from non-diabetic rats. Transplantation of ASC-rich cells from non-diabetic rats promoted wound healing in both non-diabetic and diabetic rats. In contrast, d-ASC-rich cells and enriched nuclear cells only promoted wound healing in non-diabetic rats. ASC-rich cell transplantation promoted greater tissue regeneration than d-ASC-rich cell transplantation.
ASC-rich cells promoted wound healing in diabetic and non-diabetic rats. ASC density was lower in the adipose tissue of diabetic rats compared with non-diabetic rats. d-ASC-rich cells did not promote wound healing in diabetic rats, suggesting that caution is warranted regarding the clinical use of diabetic adipose stem cell transplantation for the treatment of diabetic wounds.
本研究旨在探讨糖尿病大鼠和非糖尿病大鼠来源的脂肪组织干细胞（ASCs）在不同微环境中对伤口愈合的影响。
通过特征性表面抗原检测区分两种类型的 ASC 丰富细胞。将 ASC 丰富细胞移植到糖尿病和非糖尿病大鼠的伤口中。比较伤口愈合率，并在伤口边缘切片中观察愈合过程，以确定 ASC 丰富细胞如何在不同微环境中影响伤口愈合。
糖尿病大鼠中 ASC 的密度降低。糖尿病大鼠 ASC 丰富细胞（d-ASC 丰富细胞）的生成时间长于非糖尿病大鼠 ASC 丰富细胞。d-ASC 丰富细胞第三代（传代 3）中的凋亡前细胞数量高于非糖尿病大鼠 ASC 丰富细胞。d-ASC 丰富细胞中 CD31 和 CD34 的表达高于非糖尿病大鼠 ASC 丰富细胞，而 CD44 和 CD105 的表达低于非糖尿病大鼠 ASC 丰富细胞。非糖尿病大鼠 ASC 丰富细胞的移植促进了非糖尿病和糖尿病大鼠的伤口愈合。相比之下，d-ASC 丰富细胞和富集核细胞仅促进了非糖尿病大鼠的伤口愈合。ASC 丰富细胞的移植促进了比 d-ASC 丰富细胞移植更多的组织再生。
ASC 丰富细胞促进了糖尿病和非糖尿病大鼠的伤口愈合。与非糖尿病大鼠相比，糖尿病大鼠脂肪组织中的 ASC 密度较低。d-ASC 丰富细胞不能促进糖尿病大鼠的伤口愈合，这表明在临床应用糖尿病脂肪干细胞移植治疗糖尿病伤口时需要谨慎。
