State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science Technology, Anhui Agricultural University, Heifei, 230036, China.
State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science Technology, Anhui Agricultural University, Heifei, 230036, China.
Environ Pollut. 2021 Jul 15;281:117063. doi: 10.1016/j.envpol.2021.117063. Epub 2021 Apr 7.
This is a research comment on the ochratoxin A (OTA) degradation mechanism by Lysobacter sp. CW239 regarding the previous publication in Environmental Pollution (Wei et al., 2020). Three possible degradation mechanisms were discussed in the referred publication, but without definite evidences, it was not clear which one worked actually. Here, the gene cp4 deficient mutant CW239 was successfully constructed, and the carboxypeptidase CP4 role on OTA degradation in strain CW239 was validated in vivo. As a result, the mutant CW239 without gene cp4 showed less than 10% reduction of 24 hrs degradation ratio compared to wide-type strain CW239. After the gene cp4 complemented to CW239, the complementary strain (+)cp4 recovered the degradation ability to wide-type. The validation result indicated that the third degradation mechanism (i.e., OTA is degraded by joint action of multiple enzymes in CW239) proposed previous (Wei et al., 2020) was correct route for the degradation strain. This commentary was significant to the following studies on the pollutant detoxify strains with similar degradation characters between identified enzyme and the host strain.
这是一篇关于 Lysobacter sp. CW239 降解赭曲霉毒素 A（OTA）机制的研究评论，针对之前在《环境污染》（Wei 等人，2020 年）上的发表的文章。在之前的出版物中讨论了三种可能的降解机制，但由于没有明确的证据，不清楚实际上哪种机制起作用。在这里，成功构建了基因 cp4 缺失突变体 CW239，并在体内验证了菌株 CW239 中羧肽酶 CP4 在 OTA 降解中的作用。结果表明，与野生型菌株 CW239 相比，缺失基因 cp4 的突变体 CW239 在 24 小时的降解率降低不到 10%。当基因 cp4 被互补到 CW239 后，互补菌株 (+)cp4 恢复了对野生型的降解能力。验证结果表明，之前提出的（Wei 等人，2020 年）的第三种降解机制（即 OTA 被 CW239 中的多种酶联合作用降解）是降解菌株的正确途径。这篇评论对具有类似酶和宿主菌株降解特征的污染物解毒菌株的后续研究具有重要意义。
