Khoury Rachelle El, Mathieu Florence, Atoui Ali, Kawtharani Hiba, Khoury Anthony El, Afif Charbel, Maroun Richard G, Khoury André El
Laboratoire de Mycologie et Sécurité des Aliments (LMSA), Centre d'analyse et de Recherche (CAR), Campus des Sciences et Technologie, Université Saint-Joseph, Dekwaneh-Beyrouth 1104-2020, Lebanon.
Laboratoire de Génie Chimique, CNRS, INPT, UPS, Université de Toulouse, Toulouse 31 326, France.
Toxins (Basel). 2017 Jul 14;9(7):222. doi: 10.3390/toxins9070222.
Ochratoxin A (OTA) is one of the most important mycotoxins, and contaminates several agricultural products, particularly cereals, grapes, maize, barley, spices and coffee. The aim of this project was to reduce the levels of OTA by supplementing the artificially contaminated solutions with seven strains of actinobacteria (AT10, AT8, SN7, MS1, ML5, G10 and PT1) in order to evaluate their capacity for binding and metabolizing the OTA, as well as their ability to reduce the expression of the genes responsible for its production in . In the first part of this study, we evaluated the capacity of strains for binding OTA on their surfaces after 0, 30 and 60 min of incubation with PBS solution supplemented with OTA. In the second part, we tested the ability of these strains, as well as their supernatants, to detoxify the ISP2 medium. Finally, we studied the effect of the cocultured with on the expression of OTA biosynthesis genes. Results showed that, among the strains co-cultured with , the strain G10 was able to reduce the expression of , , and genes, thus reducing OTA from solid PDA medium to 13.50% of reduction. This strain was remarkably able to detoxify and bind OTA up to 47.07%. Strain AT8 was stronger in detoxifying OTA (52.61%), but had no significant effect on the studied gene expression.
赭曲霉毒素A（OTA）是最重要的霉菌毒素之一，会污染多种农产品，尤其是谷物、葡萄、玉米、大麦、香料和咖啡。本项目的目的是通过向人工污染的溶液中添加7株放线菌（AT10、AT8、SN7、MS1、ML5、G10和PT1）来降低OTA水平，以评估它们结合和代谢OTA的能力，以及它们降低负责其产生的基因在……中表达的能力。在本研究的第一部分，我们评估了在与添加了OTA的PBS溶液孵育0、30和60分钟后，这些菌株在其表面结合OTA的能力。在第二部分中，我们测试了这些菌株及其上清液对ISP2培养基解毒的能力。最后，我们研究了与……共培养对OTA生物合成基因表达的影响。结果表明，在与……共培养的菌株中，G10菌株能够降低……、……、……和……基因的表达，从而使固体PDA培养基中的OTA降低至13.50%。该菌株能够显著地解毒并结合OTA，高达47.07%。AT8菌株在解毒OTA方面更强（52.61%），但对所研究的基因表达没有显著影响。
