基于 LCR 的乙型肝炎不同谱患者热点 G1896A 突变的快速检测。
LCR based quick detection of hotspot G1896A mutation in patients with different spectrum of hepatitis B.
机构信息
Nanobiotechnology Unit, Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Department of Clinical Laboratory Science, College of Applied Medical Science, King Saud University, Riyadh, 11564, Saudi Arabia; Prince Sattam Chair for Epidemiology and Public Health Research, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
出版信息
J Infect Public Health. 2021 May;14(5):651-654. doi: 10.1016/j.jiph.2021.01.013. Epub 2021 Jan 28.
G1896A switch is one of the hotspots in subjects affected with hepatitis B. This hotspot mutation is observed in all the different spectrum of hepatitis B, and it has a very dangerous and a long lasting effect. The major purpose of the study was to screen G1986A mutations at a large scale and also to establish ligase chain reaction as a mutation testing tool. Polymerase chain reaction (PCR) and Nucleotide Sequencing was done to identify the G1896A mutation in the precore region of the genome. All the 331 HBV positive patients were screened. Almost 29% (24/82) of the cases remarkably had the presence of G1896A mutation confirmed by LCR and direct sequencing. The precore G1896A mutation is responsible for one third of the patients suffering from precore stop codon mutation. It clearly exhibits that LCR is 100% in sync with direct sequencing and is extremely reliable and the results are highly reproducible.
G1896A 突变是乙型肝炎相关研究的热点之一。该热点突变存在于乙型肝炎的所有不同谱中，具有非常危险且持久的影响。本研究的主要目的是大规模筛选 G1986A 突变，并建立连接酶链反应作为突变检测工具。聚合酶链反应（PCR）和核苷酸测序用于鉴定基因组前核心区的 G1896A 突变。对所有 331 例 HBV 阳性患者进行了筛选。通过 LCR 和直接测序证实，近 29%（24/82）的病例明显存在 G1896A 突变。前核心区 G1896A 突变是导致三分之一的患者出现前核心终止密码子突变的原因。这清楚地表明 LCR 与直接测序完全一致，并且非常可靠，结果高度可重复。
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