Malhotra S K, Svensson M, Aldskogius H, Bhatnagar R, Das G D, Shnitka T K
Department of Zoology, University of Alberta, Edmonton, Canada.
Brain Res Bull. 1993;30(3-4):395-404. doi: 10.1016/0361-9230(93)90271-c.
An Astrocyte-specific antigen recognized by monoclonal antibody J1-31 is a more intense marker for proximal reactive astrocytes in lacerated rat spinal cord than is glial fibrillary acidic protein (GFAP). Thus, MAb J1-31 recognizes reactive astrocytes in the immediate vicinity of the lesion, whereas reactive astrocytes that are located at a distance from the lesion are not detected by immunofluorescent staining. These findings are relevant to the biochemical heterogeneity manifested respectively by reactive astrocytes located proximal and distal to a laceration-type injury of the spinal cord, and those that develop following axotomy with retrograde degeneration. Reactive astrocytes in the axotomy model are not stained with MAb J1-31, but are positive for GFAP.
单克隆抗体J1-31识别的一种星形胶质细胞特异性抗原，对于大鼠脊髓损伤中近端反应性星形胶质细胞来说，是比胶质纤维酸性蛋白（GFAP）更强的标志物。因此，单克隆抗体J1-31识别损伤紧邻区域的反应性星形胶质细胞，而距损伤较远的反应性星形胶质细胞则不能通过免疫荧光染色检测到。这些发现与脊髓撕裂伤型损伤近端和远端的反应性星形胶质细胞，以及轴突切断伴逆行性变性后产生的反应性星形胶质细胞分别表现出的生化异质性相关。轴突切断模型中的反应性星形胶质细胞不能被单克隆抗体J1-31染色，但对GFAP呈阳性。
