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大肠杆菌外膜中蛋白质I的排列：交联研究

Arrangement of protein I in Escherichia coli outer membrane: cross-linking study.

作者信息

Palva E T, Randall L L

出版信息

J Bacteriol. 1978 Jan;133(1):279-86. doi: 10.1128/jb.133.1.279-286.1978.

DOI:10.1128/jb.133.1.279-286.1978
PMID:338582
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC222005/
Abstract

The arrangement of protein I in the outer membrane of Escherichia coli was investigated by cross-linking whole cells, isolated cell wall, protein-peptidoglycan complexes, and protein I released from peptidoglycan with NaCl. Both cleavable azide cross-linkers and imidoester reagents were used. The data presented suggest that protein I exists in the outer membrane as a trimer.

摘要

通过对完整细胞、分离的细胞壁、蛋白质-肽聚糖复合物以及用氯化钠从肽聚糖中释放的蛋白质I进行交联，研究了蛋白质I在大肠杆菌外膜中的排列方式。使用了可裂解的叠氮化物交联剂和亚胺酯试剂。所呈现的数据表明，蛋白质I在外膜中以三聚体形式存在。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e89b/222005/32b59978c1df/jbacter00296-0302-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e89b/222005/d0a3162dd47b/jbacter00296-0298-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e89b/222005/910866d6200c/jbacter00296-0299-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e89b/222005/4cda53e40b06/jbacter00296-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e89b/222005/639be722c687/jbacter00296-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e89b/222005/32b59978c1df/jbacter00296-0302-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e89b/222005/d0a3162dd47b/jbacter00296-0298-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e89b/222005/910866d6200c/jbacter00296-0299-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e89b/222005/4cda53e40b06/jbacter00296-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e89b/222005/639be722c687/jbacter00296-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e89b/222005/32b59978c1df/jbacter00296-0302-a.jpg

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本文引用的文献

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Tables for estimating sedimentation through linear concentration gradients of sucrose solution.用于通过蔗糖溶液线性浓度梯度估算沉降的表格。
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Use of dimethyl suberimidate, a cross-linking reagent, in studying the subunit structure of oligomeric proteins.
大肠杆菌B/r主要外膜蛋白I（ompF蛋白，孔蛋白）的一级结构
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Determination of ion permeability through the channels made of porins from the outer membrane of Salmonella typhimurium in lipid bilayer membranes.鼠伤寒沙门氏菌外膜孔蛋白构成的通道在脂质双分子层膜中离子通透性的测定。
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Cross-reactivity of major outer membrane proteins of Enterobacteriaceae, studied by crossed immunoelectrophoresis.通过交叉免疫电泳研究肠杆菌科主要外膜蛋白的交叉反应性。
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Outer membrane proteins of Brucella abortus: isolation and characterization.流产布鲁氏菌外膜蛋白：分离与鉴定
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