李斯特菌 S2542 全基因组序列及其在超高压处理下部分基因的表达情况。
The complete genome sequence of Listeria monocytogenes strain S2542 and expression of selected genes under high-pressure processing.
机构信息
Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
Faculty of Food Science and Engineering, Dunarea de Jos University of Galati, Galati, Romania.
出版信息
BMC Res Notes. 2021 Apr 15;14(1):137. doi: 10.1186/s13104-021-05555-2.
OBJECTIVES
The study aims to generate the whole genome sequence of L. monocytogenes strain S2542 and to compare it to the genomes of strains RO15 and ScottA. In addition, we aimed to compare gene expression profiles of L. monocytogenes strains S2542, ScottA and RO15 after high-pressure processing (HPP) using ddPCR.
RESULTS
The whole genome sequence of L. monocytogenes S2542 indicates that this strain belongs to serotype 4b, in contrast to the previously reported serotype 1/2a. Strain S2542 appears to be more susceptible to the treatment at 400 MPa compared to RO15 and ScottA strains. In contrast to RO15 and ScottA strains, viable cell counts of strain S2542 were below the limit of detection after HPP (400 MPa/8 min) when stored at 8 °C for 24 and 48 h. The transcriptional response of all three strains to HPP was not significantly different.
目的
本研究旨在生成李斯特菌 S2542 菌株的全基因组序列，并将其与 RO15 和 ScottA 菌株的基因组进行比较。此外，我们还旨在使用 ddPCR 比较高压处理 (HPP) 后李斯特菌 S2542、ScottA 和 RO15 菌株的基因表达谱。
结果
李斯特菌 S2542 的全基因组序列表明，该菌株属于 4b 血清型，与之前报道的 1/2a 血清型不同。与 RO15 和 ScottA 菌株相比，S2542 菌株在 400 MPa 下似乎更容易受到处理的影响。与 RO15 和 ScottA 菌株不同，当在 8°C 下储存 24 和 48 小时时，S2542 菌株的活菌计数在 HPP（400 MPa/8 min）后低于检测限。三种菌株对 HPP 的转录反应没有显著差异。
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