参与重组刺激因子产生、对某些损伤因子敏感性控制以及大肠杆菌K-12 HfrC中染色体复制的F质粒基因。
F plasmid genes involved in the production of recombination-stimulating factor, control of sensitivity to some injurious agents, and chromosome replication in Escherichia coli K-12 HfrC.
作者信息
Chernin L S, Ovadis M I, Goldfarb D M
出版信息
J Bacteriol. 1978 Jan;133(1):33-42. doi: 10.1128/jb.133.1.33-42.1978.
Abstract
Three related F'arg+ plasmids isolated by Guyer and Clark were used to analyze some properties of strain MG751, a recipient derivative of an HfrC mutant (MG7) carrying a previously described pleiotropic mutation in the integrated F plasmid. Strains MG7 and MG751 both failed to produce recombination-stimulating factor, were sensitive to monofunctional alkylating agents and UV irradiation, and were temperature sensitive for growth and DNA synthesis. It was shown that these phenotypes are controlled by F plasmids genes (designated rsf, prt, and rep) that can be separated by deletion mutations occurring on the F plasmid.
摘要
盖耶尔和克拉克分离出的三种相关的F'arg+质粒被用于分析菌株MG751的一些特性，MG751是HfrC突变体（MG7）的受体衍生物，其整合的F质粒中携带一个先前描述的多效性突变。菌株MG7和MG751都不能产生重组刺激因子，对单功能烷化剂和紫外线照射敏感，并且对生长和DNA合成具有温度敏感性。结果表明，这些表型由F质粒基因（命名为rsf、prt和rep）控制，这些基因可通过F质粒上发生的缺失突变而分离。
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