Institut de Recherche Biomédicale des Armées, Equipe de Recherche Subaquatique Opérationnelle, 83800, Toulon Cedex 9, France.
Univ Brest, ORPHY, IBSAM, 29200, Brest, France.
Sci Rep. 2021 Apr 15;11(1):8317. doi: 10.1038/s41598-021-87952-y.
On one side, decompression sickness (DCS) with neurological disorders lead to a reshuffle of the fecal metabolome from rat caecum. On the other side, there is high inter-individual variability in terms of occurrence of DCS. One could wonder whether the fecal metabolome could be linked to the DCS-susceptibility. We decided to study male and female rats selected for their resistance to decompression sickness, and we hypothesize a strong impregnation concerning the fecal metabolome. The aim is to verify whether the rats resistant to the accident have a fecal metabolomic signature different from the stem generations sensitive to DCS. 39 DCS-resistant animals (21 females and 18 males), aged 14 weeks, were compared to 18 age-matched standard Wistar rats (10 females and 8 males), i.e., the same as those we used for the founding stock. Conventional and ChemRICH approaches helped the metabolomic interpretation of the 226 chemical compounds analyzed in the cecal content. Statistical analysis shows a panel of 81 compounds whose expression had changed following the selection of rats based on their resistance to DCS. 63 compounds are sex related. 39 are in common. This study shows the spectral fingerprint of the fecal metabolome from the caecum of a strain of rats resistant to decompression sickness. This study also confirms a difference linked to sex in the metabolome of non-selected rats, which disappear with selective breeding. Results suggest hormonal and energetic reshuffle, including steroids sugars or antibiotic compounds, whether in the host or in the microbial community.
一方面，伴有神经系统障碍的减压病（DCS）导致大鼠盲肠粪便代谢组发生重新洗牌。另一方面，DCS 的发生存在高度的个体间变异性。人们可能会想，粪便代谢组是否与 DCS 易感性有关。我们决定研究对减压病有抵抗力的雄性和雌性大鼠，并假设粪便代谢组有强烈的遗传渗透。目的是验证对事故有抵抗力的大鼠的粪便代谢组是否与对 DCS 敏感的亲代种群有不同的特征。将 39 只抗减压病的动物（21 只雌性和 18 只雄性）与 18 只年龄匹配的标准 Wistar 大鼠（10 只雌性和 8 只雄性）进行比较，即与用于建立种群的大鼠相同。常规和 ChemRICH 方法有助于解释在盲肠内容物中分析的 226 种化学化合物的代谢组学。统计分析显示，有一组 81 种化合物的表达在根据其对 DCS 的抵抗力对大鼠进行选择后发生了变化。其中 63 种与性别有关。有 39 种是共同的。这项研究显示了抗减压病大鼠盲肠粪便代谢组的光谱指纹。这项研究还证实了非选择性大鼠代谢组中与性别有关的差异，这种差异在选择性繁殖后消失。结果表明，包括类固醇、糖或抗生素化合物在内的激素和能量的重新调整，无论是在宿主中还是在微生物群落中。
