Dugrenot Emmanuel, Guernec Anthony, Orsat Jérémy, Guerrero François
Univ Brest, ORPHY's Laboratory, 6 Av Le Gorgeu, CS93837, F-29238, Brest, Cedex, France.
Tek Diving SAS, Brest, France.
Commun Biol. 2024 Oct 2;7(1):1245. doi: 10.1038/s42003-024-06963-3.
Susceptibility to decompression sickness (DCS) is characterized by a wide inter-individual variability, the origins of which are still poorly understood. We selectively bred rats with at least a 3-fold greater resistance to DCS than standard rats after 6 generations. In order to better understand DCS mechanisms, we compared the static genome expression of these resistant rats from the 10 generation to their counterparts of the initial non-resistant Wistar strain, by a microarray transcriptomic approach coupled and crossed with a PCR plates miRnome study. Thus, we identified differentially expressed genes on selected males and females, as well as gender differences in those genes, and we crossed these transcripts with the respective targets of the differentially expressed microRNAs. Our results highlight pathways involved in inflammatory responses, circadian clock, cell signaling and motricity, phagocytosis or apoptosis, and they confirm the importance of inflammation in DCS pathophysiology.
减压病（DCS）易感性的特点是个体间差异很大，其根源仍知之甚少。经过6代选育，我们培育出了对DCS的抵抗力比标准大鼠至少高3倍的大鼠。为了更好地理解DCS机制，我们通过与PCR板miRnome研究相结合的微阵列转录组学方法，比较了这些抗性大鼠第10代与初始非抗性Wistar品系对应大鼠的静态基因组表达。因此，我们确定了选定雄性和雌性大鼠中差异表达的基因，以及这些基因中的性别差异，并将这些转录本与差异表达的微小RNA的各自靶标进行了交叉分析。我们的结果突出了参与炎症反应、昼夜节律时钟、细胞信号传导和运动性、吞噬作用或细胞凋亡的途径，并证实了炎症在DCS病理生理学中的重要性。
