Peng Dan, Hou Zi-Liang, Zhang Hong-Xia, Zhang Shuai, Zhang Shu-Ming, Lin Rui-Yan, Xing Zhen-Chuan, Yuan Yuan, Yang Kai-Yuan, Wang Jin-Xiang
Department of Respiratory and Critical Care Medicine, Beijing Luhe Hospital, Capital Medical University, Beijing, People's Republic of China.
Int J Gen Med. 2021 Apr 9;14:1239-1249. doi: 10.2147/IJGM.S304199. eCollection 2021.
Pulmonary thromboembolism (PTE) is a common disease which may be a serious condition and has high mortality. Recently, it has been shown that circRNAs play an important role in the development of various diseases, including thromboembolic disease. However, circRNAs expression profiling is not clear in PTE, this study aims to identify the circRNAs expressed in PTE and to elucidate their possible role in pathophysiology of PTE.
A total of 5 patients with CTPA-confirmed PTE and 5 healthy controls were recruited for the present study. The circRNAs expression profile was analyzed by microarray.
In total, 256 differentially expressed circRNAs (up 142, down114) and 1162 mRNA (up 446, down 716) were summarized by analyzing the circRNAs microarray data. The top 3 up-regulated and 3 down-regulated circRNAs were validated by Real-Time Polymerase Chain Reaction (qRT-PCR). Two differentially expressed circRNAs (hsa_circ_0000891, hsa_circ_0043506) were selected for further analysis. Finally, we construct a circRNA-miRNA-mRNA ceRNA network with a bioinformatic prediction tool. Pathway analysis shows that the enriched mRNAs targets take part in Protein processing in endoplasmic reticulum, Systemic lupus erythematosus, Endocytosis, Spliceosome, HTLV-I infection and Ubiquitin mediated proteolysis.
Our findings indicated that aberrantly expressed circRNAs (hsa_circ_0000891, hsa_circ_0043506) may be involved in the development of PTE.
肺血栓栓塞症（PTE）是一种常见疾病，可能病情严重且死亡率高。最近研究表明，环状RNA（circRNAs）在包括血栓栓塞性疾病在内的多种疾病发展中发挥重要作用。然而，PTE中circRNAs的表达谱尚不清楚，本研究旨在鉴定PTE中表达的circRNAs，并阐明它们在PTE病理生理学中的可能作用。
本研究共纳入5例经CTPA确诊的PTE患者和5名健康对照。通过微阵列分析circRNAs表达谱。
通过分析circRNAs微阵列数据，共总结出256个差异表达的circRNAs（上调142个，下调114个）和1162个mRNA（上调446个，下调716个）。通过实时聚合酶链反应（qRT-PCR）验证了上调和下调排名前3的circRNAs。选择两个差异表达的circRNAs（hsa_circ_0000891，hsa_circ_0043506）进行进一步分析。最后，我们使用生物信息学预测工具构建了一个circRNA- miRNA-mRNA竞争性内源RNA（ceRNA）网络。通路分析表明，富集的mRNA靶标参与内质网中的蛋白质加工、系统性红斑狼疮、内吞作用、剪接体、人类嗜T淋巴细胞病毒I型感染和泛素介导的蛋白水解。
我们的研究结果表明，异常表达的circRNAs（hsa_circ_0000891，hsa_circ_0043506）可能参与PTE的发展。
