Department of Parasitology, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
Parasit Vectors. 2021 Sep 21;14(1):484. doi: 10.1186/s13071-021-04996-9.
This letter responds to comments on our article (Yin YL et al., Parasit Vectors, 10.1186/s13071-021-04739-w) by Yuqing Wang and colleagues, who wrote a letter entitled "Microarray analysis of circular RNAs in HCT-8 cells infected with Cryptosporidium parvum" and discussed statistical procedures for microarray analysis during C. parvum infection. To further confirm our data, in this letter, a common R package for analyses of differentially expressed genes, namely DESeq2, with Benjamini-Hochberg correction, was used to analyze our microarray data and identified 26 significantly differentially expressed circRNAs using adjusted P value < 0.05 and | Log (fold change [FC]) | ≥ 1.0, including our circRNA ciRS-7 of interest. Therefore, the protocol for selecting circRNAs of interest for further study in our article is acceptable and did not affect the subsequent scientific findings in our article.
这封回信是对王玉清等作者来信的回复，来信题为“微小隐孢子虫感染 HCT-8 细胞的环状 RNA 的微阵列分析”，讨论了微小隐孢子虫感染过程中的微阵列分析的统计程序。为了进一步证实我们的数据，在这封回信中，我们使用了一种常见的差异表达基因分析的 R 包 DESeq2，并结合 Benjamini-Hochberg 校正，分析了我们的微阵列数据，鉴定了 26 个差异表达的显著差异环状 RNA，采用调整后的 P 值<0.05 和 |Log（fold change [FC]）|≥1.0，包括我们感兴趣的环状 RNA ciRS-7。因此，我们文章中选择进一步研究的环状 RNA 的方案是可以接受的，并没有影响我们文章中的后续科学发现。
