Yin Qiaoxiu, Yang Rui, Ren Yafeng, Yang Zhiying, Li Tao, Huang Honglin, Tang Qin, Li Dongxue, Jiang Shilong, Wu Xian, Wang Delu, Chen Zhuo
Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, China.
College of Agricultural, Guizhou University, Guiyang, China.
Front Microbiol. 2021 Mar 30;12:618476. doi: 10.3389/fmicb.2021.618476. eCollection 2021.
Gray blight disease is one of the most destructive diseases of tea plants and occurs widely in the tea-growing areas of the world. It is caused by several fungal phytopathogens, of which is the main pathogen in China. The environmentally friendly antimicrobial, phenazine-1-carboxylic acid (PCA), a metabolite of the natural soil-borne bacteria spp., can inhibit a range of fungal crop diseases. In this study, we determined that PCA was active against We studied the mode of action of PCA on hyphae using a microscopic investigation, transcriptomics, biochemical methods, and molecular docking. The results of scanning and transmission electron microscopy indicated that PCA caused developmental deformity of mycelia and organelle damage, and it significantly decreased the accumulation of exopolysaccharides on the hyphal surface. The transcriptome revealed that 1705 and 1683 differentially expressed genes of treated with PCA were up-regulated or down-regulated, respectively, with genes associated with ribosome biogenesis, oxidative phosphorylation, and encoding various proteins of -glycan biosynthesis being significantly up-regulated. Up-regulation of nine genes related to -glycan biosynthesis of in response to PCA treatment was confirmed by reverse transcription qPCR. The enzymatic activity of catalase and superoxide dismutase of hyphae was significantly decreased by PCA treatment. Our results indicated that exposure to PCA resulted in expression changes in oxidoreductase genes, accumulation of reactive oxygen species, and decreased activity of catalase, with concomitant damage to the fungal cell membrane and cell wall.
灰枯病是茶树最具破坏性的病害之一，在世界各产茶区广泛发生。它由多种真菌植物病原体引起，其中在中国主要病原体是[此处原文缺失相关内容]。环境友好型抗菌剂吩嗪 - 1 - 羧酸（PCA）是天然土壤细菌[此处原文缺失相关内容]属的一种代谢产物，可抑制多种真菌作物病害。在本研究中，我们确定PCA对[此处原文缺失相关内容]有活性。我们使用显微镜观察、转录组学、生化方法和分子对接研究了PCA对菌丝的作用模式。扫描电子显微镜和透射电子显微镜结果表明，PCA导致菌丝体发育畸形和细胞器损伤，并显著降低菌丝表面胞外多糖的积累。转录组显示，用PCA处理的[此处原文缺失相关内容]分别有1705个和1683个差异表达基因上调或下调，与核糖体生物发生、氧化磷酸化以及编码各种β - 聚糖生物合成蛋白相关的基因显著上调。通过逆转录qPCR证实了响应PCA处理的[此处原文缺失相关内容]中九个与β - 聚糖生物合成相关基因的上调。PCA处理显著降低了菌丝的过氧化氢酶和超氧化物歧化酶的酶活性。我们的结果表明，接触PCA导致氧化还原酶基因表达变化、活性氧积累以及过氧化氢酶活性降低，同时对真菌细胞膜和细胞壁造成损伤。
