Gegunde Sandra, Alfonso Amparo, Alvariño Rebeca, Alonso Eva, Botana Luis M
Departamento de Farmacología, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo, Spain.
Grupo Investigación Biodiscovery, Instituto de Investigación Sanitaria de Santiago de Compostela, Santiago de Compostela, Spain.
Front Immunol. 2021 Mar 30;12:609196. doi: 10.3389/fimmu.2021.609196. eCollection 2021.
Cyclophilins (Cyps) are a group of peptidyl-prolyl isomerases that play crucial roles in regulatory mechanisms of cellular physiology and pathology in several inflammatory conditions. Their receptor, CD147, also participates in the development and progression of the inflammatory response. Nevertheless, the main function of Cyps and their receptor are yet to be deciphered. The release of CypA and the expression of the CD147 receptor in activated T lymphocytes were already described, however, no data are available about other Cyps in these cells. Therefore, in the present work intra and extracellular CypA, B and C levels were measured followed by induced inflammatory conditions. After activation of T lymphocytes by incubation with concanavalin A, both intra and extracellular Cyps levels and the CD147 membrane receptor expression were increased leading to cell migration towards circulating CypA and CypB as chemoattractants. When CypA was modulated by natural and synthetic compounds, the inflammatory cascade was avoided including T cell migration. Our results strengthen the relationship between CypA, B, and C, their receptor, and the inflammatory process in human T lymphocytes, associating CypC with these cells for the first time.
亲环蛋白（Cyps）是一类肽基脯氨酰异构酶，在多种炎症状态下的细胞生理和病理调节机制中发挥关键作用。它们的受体CD147也参与炎症反应的发生和发展。然而，Cyps及其受体的主要功能尚未完全阐明。此前已有关于活化T淋巴细胞中CypA的释放及CD147受体表达的报道，但尚无这些细胞中其他Cyps的相关数据。因此，在本研究中，我们测定了诱导炎症状态下细胞内和细胞外CypA、B和C的水平。在用伴刀豆球蛋白A孵育激活T淋巴细胞后，细胞内和细胞外Cyps水平以及CD147膜受体表达均增加，导致细胞向循环中的CypA和CypB趋化迁移，CypA和CypB作为趋化因子。当用天然和合成化合物调节CypA时，可避免炎症级联反应，包括T细胞迁移。我们的结果强化了CypA、B和C及其受体与人类T淋巴细胞炎症过程之间的关系，首次将CypC与这些细胞联系起来。
