Lu Yuntao, Liu Bing, Ma Junjie, Yang Shuo, Huang Ju
Department of Pulmonary and Critical Care Medicine, Huadong Hospital, Fudan University, Shanghai, China.
Center for Brain Science, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Front Genet. 2021 Mar 30;12:664334. doi: 10.3389/fgene.2021.664334. eCollection 2021.
Inadequate sleep prevails in modern society and it impairs the circadian transcriptome. However, to what extent acute sleep deprivation (SD) has impact on the circadian rhythms of peripheral tissues is not clear. Here, we show that in mouse lung, a 10-h acute sleep deprivation can alter the circadian expression of approximately 3,000 genes. We found that circadian rhythm disappears in genes related to metabolism and signaling pathways regulating protein phosphorylation after acute sleep deprivation, while the core circadian regulators do not change much in rhythmicity. Importantly, the strong positive correlation between mean expression and amplitude (E-A correlation) of cycling genes has been validated in both control and sleep deprivation conditions, supporting the energetic cost optimization model of circadian gene expression. Thus, we reveal that acute sleep deprivation leads to a profound change in the circadian gene transcription that influences the biological functions in lung.
睡眠不足在现代社会中普遍存在，它会损害昼夜转录组。然而，急性睡眠剥夺（SD）对外周组织昼夜节律的影响程度尚不清楚。在此，我们表明，在小鼠肺中，10小时的急性睡眠剥夺可改变约3000个基因的昼夜表达。我们发现，急性睡眠剥夺后，与调节蛋白质磷酸化的代谢和信号通路相关的基因的昼夜节律消失，而核心昼夜调节因子的节律性变化不大。重要的是，在对照和睡眠剥夺条件下，循环基因的平均表达与振幅之间的强正相关（E-A相关性）均得到验证，支持昼夜基因表达的能量成本优化模型。因此，我们揭示了急性睡眠剥夺导致昼夜基因转录发生深刻变化，从而影响肺中的生物学功能。
