Department of Forensic Molecular Biology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands.
Chronobiol Int. 2013 Aug;30(7):901-9. doi: 10.3109/07420528.2013.784773. Epub 2013 Jun 5.
This study investigated the impact of sleep deprivation on the human circadian system. Plasma melatonin and cortisol levels and leukocyte expression levels of 12 genes were examined over 48 h (sleep vs. no-sleep nights) in 12 young males (mean±SD: 23±5 yrs). During one night of total sleep deprivation, BMAL1 expression was suppressed, the heat shock gene HSPA1B expression was induced, and the amplitude of the melatonin rhythm increased, whereas other high-amplitude clock gene rhythms (e.g., PER1-3, REV-ERBα) remained unaffected. These data suggest that the core clock mechanism in peripheral oscillators is compromised during acute sleep deprivation.
这项研究调查了睡眠剥夺对人体昼夜节律系统的影响。在 12 名年轻男性（平均年龄±标准差：23±5 岁）中，在 48 小时内（睡眠与非睡眠夜晚）检测了血浆褪黑素和皮质醇水平以及 12 个基因的白细胞表达水平。在一整夜的完全睡眠剥夺期间，BMAL1 表达受到抑制，热休克基因 HSPA1B 的表达被诱导，并且褪黑素节律的幅度增加，而其他高幅度时钟基因节律（例如，PER1-3、REV-ERBα）不受影响。这些数据表明，在急性睡眠剥夺期间，外周振荡器中的核心时钟机制受损。
