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接受急诊腹部手术的无症状患者腹膜拭子中的新型冠状病毒2型

SARS-CoV-2 in peritoneal swabs from asymptomatic patients undergoing emergency abdominal surgery.

作者信息

AlAradi Jasim, AlHarmi Rawan A Rahman, AlKooheji Mariam, Almahari Sayed Ali, Isa Mohamed Abdulla, AlMarzooq Raed

机构信息

Department of Surgery, Salmaniya Medical Complex, Manama, Bahrain.

Department of Pathology, Salmaniya Medical Complex, Manama, Bahrain.

出版信息

J Surg Case Rep. 2021 Apr 9;2021(4):rjab116. doi: 10.1093/jscr/rjab116. eCollection 2021 Apr.

Abstract

This is a case series of five patients with acute abdomen requiring surgery who tested positive for coronavirus disease 2019 (COVID-19) and were asymptomatic, with the purpose of detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in peritoneal fluid. Nasopharyngeal swab was done as a prerequisite for admission or prior to admission as part of random testing. Two methods of viral testing were employed: Xpert® Xpress SARS-CoV-2 (rapid test) and real-time reverse transcription polymerase chain reaction (RT-PCR). Either or both tests were done, with the former performed for patients requiring surgery immediately. Surgery was performed within 24-36 h from admission. Peritoneal fluid swabs were obtained for the detection of SARS-CoV-2 using RT-PCR test. Swabs were immediately placed in viral transfer media and delivered to the public health laboratory in an ice bag. SARS-CoV-2 was not detected in peritoneal swabs. Due to the limited number of patients, further studies are required; yet, protective measures should still be taken by surgeons when dealing with COVID-19 cases.

摘要

这是一组五例需要手术治疗的急腹症患者的病例系列,这些患者新型冠状病毒肺炎(COVID-19)检测呈阳性且无症状,目的是检测腹膜液中的严重急性呼吸综合征冠状病毒2(SARS-CoV-2)。入院前或入院时作为随机检测的一部分进行鼻咽拭子检查是入院的前提条件。采用了两种病毒检测方法:Xpert® Xpress SARS-CoV-2(快速检测)和实时逆转录聚合酶链反应(RT-PCR)。两种检测方法任选其一或同时进行,前者用于需要立即手术的患者。入院后24至36小时内进行手术。使用RT-PCR检测法获取腹膜液拭子以检测SARS-CoV-2。拭子立即放入病毒转运培养基中,并装在冰袋中送至公共卫生实验室。腹膜拭子中未检测到SARS-CoV-2。由于患者数量有限,需要进一步研究;然而,外科医生在处理COVID-19病例时仍应采取保护措施。

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