大肠杆菌外膜脂蛋白结构基因的基因剂量效应。
Gene dosage effects of the structural gene for a lipoprotein of the Escherichia coli outer membrane.
作者信息
Movva N R, Katz E, Asdourian P L, Hirota Y, Inouye M
出版信息
J Bacteriol. 1978 Jan;133(1):81-4. doi: 10.1128/jb.133.1.81-84.1978.
Abstract
The gene dosage effects of the structural gene (lpp) for the lipoprotein of the Escherichia coli outer membrane were examined. A novel F-prime factor containing the lpp gene was constructed. The amount of the free-form lipoprotein in the merodiploid strain carrying the F-prime factor was found to be about two times as great as that in the corresponding haploid strain. On the other hand, the amount of the bound-form lipoprotein, which is vovalently linked to the peptidoglycan, was not significantly different in the merodiploid strain as compared with the corresponding haploid strain. The present results suggest that the lpp gene is expressed constitutively in contrast to another major protein of the E. coli outer membrane, tolG protein (protein II, D. B. Datta et al., J. Bacteriol. 128:834-841, 1976). The F-prime factor isolated may include a portion of the E. coli chromosome (located between 33 and 36 min on the genetic map) that is not covered by any other F-prime factor.
摘要
对大肠杆菌外膜脂蛋白的结构基因（lpp）的基因剂量效应进行了研究。构建了一种含有lpp基因的新型F-prime因子。发现携带F-prime因子的部分二倍体菌株中游离形式脂蛋白的量约为相应单倍体菌株的两倍。另一方面，与肽聚糖共价连接的结合形式脂蛋白的量在部分二倍体菌株中与相应单倍体菌株相比没有显著差异。目前的结果表明，与大肠杆菌外膜的另一种主要蛋白质tolG蛋白（蛋白质II，D.B.达塔等人，《细菌学杂志》128:834 - 841，1976）相比，lpp基因是组成型表达的。分离出的F-prime因子可能包含大肠杆菌染色体的一部分（位于遗传图谱上33至36分钟之间），这部分未被任何其他F-prime因子覆盖。
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