Ahn Yejin, Kim Singeun, Suh Hyung Joo, Jung Eun Young
Department of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, Korea.
Department of Home Economic Education, Jeonju University, Jeonbuk 55069, Korea.
Prev Nutr Food Sci. 2021 Mar 31;26(1):75-81. doi: 10.3746/pnf.2021.26.1.75.
This study aimed at investigating the acute and subacute oral toxicity of yeast hydrolysate (DNF-10) in Sprague-Dawley rats. To determine the acute toxicity of DNF-10, it was orally administered to rats at a dose of 5,000 mg/kg. Furthermore, to determine subacute toxicity of DNF-10, it was orally administered to female and male rats at a dose of 1,000 mg/kg for 90 days. In the acute toxicity test, the experimental group did not show changes in body and organ weights compared to the corresponding weights in the control group. When compared to female controls, the DNF-10-treated female rats showed decreased red blood cell counts, haemoglobin, and total bilirubin levels; however, the parameters remained within the normal range. In the subacute toxicity test, DNF-10 administration significantly reduced body weight among male rats, which appears attributable to the anti-obesity effect of the compound. In the experimental groups, significant differences in some haematological parameters were considered as non-toxic because the results were within the normal range. These results suggest that DNF-10 is safe and non-toxic at single doses (5,000 mg/kg) and repeated doses (1,000 mg/kg).
本研究旨在调查酵母水解物（DNF-10）对斯普拉格-道利大鼠的急性和亚急性经口毒性。为确定DNF-10的急性毒性，以5000mg/kg的剂量对大鼠进行经口给药。此外，为确定DNF-10的亚急性毒性，以1000mg/kg的剂量对雌性和雄性大鼠进行经口给药，持续90天。在急性毒性试验中，与对照组相应体重相比，实验组的体重和器官重量未出现变化。与雌性对照组相比，接受DNF-10治疗的雌性大鼠红细胞计数、血红蛋白和总胆红素水平降低；然而，这些参数仍在正常范围内。在亚急性毒性试验中，给予DNF-10显著降低了雄性大鼠的体重，这似乎归因于该化合物的抗肥胖作用。在实验组中，一些血液学参数的显著差异被认为无毒，因为结果在正常范围内。这些结果表明，DNF-10在单剂量（5000mg/kg）和重复剂量（1000mg/kg）下是安全无毒的。
