Laboratory of Parasitic Diseases, Federal University of São Francisco Valley, Petrolina, Pernambuco, Brazil.
Collegiate of Pharmacy, Federal University of São Francisco Valley, Petrolina, Pernambuco, Brazil.
Exp Appl Acarol. 2021 May;84(1):263-270. doi: 10.1007/s10493-021-00611-9. Epub 2021 Apr 15.
The frequent use of acaricides against the tick Rhipicephalus microplus increases the risk of development of resistance. Recent studies have revealed that Neoglaziovia variegata, an indigenous plant species known in Brazil as 'caroá', has a deleterious effect against R. microplus. In the current study, extracts of N. variegata were studied for their possible acaricidal properties. A hexane extract of N. variegata leaves was fractionated in a chromatography column and the fractions were tested in adult tick immersion tests in triplicate using three concentrations (5, 10 and 25 mg/ml). All the fractions had harmful effects on the ticks. However, three fractions were more efficaceous. Phytochemical analysis indicated that stigmast-5-en-3-ol and stigmastanol were most abundant; they might be responsible for the acaricidal effects, making them potentially useful as alternative agents to control the tick R. microplus.
频繁使用杀蜱剂来防治革蜱（Rhipicephalus microplus）会增加产生抗药性的风险。最近的研究表明，巴西本土植物物种 Neoglaziovia variegata（俗称“caroá”）对革蜱具有有害影响。在本研究中，研究了 Neoglaziovia variegata 的提取物是否具有杀蜱特性。用色谱柱对 Neoglaziovia variegata 叶的己烷提取物进行了分级，并用三种浓度（5、10 和 25mg/ml）在三重复的成蜱浸浴试验中测试了各个级分。所有级分对蜱都有有害影响。然而，有三个级分更有效。植物化学分析表明，麦角甾-5-烯-3β-醇和麦角甾醇含量最丰富；它们可能是杀蜱作用的原因，使它们有可能成为控制革蜱 R. microplus 的替代药剂。
