Thorsteinsdóttir Unnur Arna, Thorsteinsdóttir Margrét
Faculty of Pharmaceutical Sciences, University of Iceland, Reykjavik, Iceland.
ArcticMass ehf., Reykjavík, Iceland.
J Mass Spectrom. 2021 Apr 2;56(9):e4727. doi: 10.1002/jms.4727.
Design of experiments (DoE) is a valuable tool for the optimization of quantitative bioanalytical methods utilizing liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Liquid chromatography mass spectrometry (LC-MS) is composed of several processes, including, liquid introduction and analyte ionization. The goal is to transfer analytes from atmospheric pressure to vacuum and maintain conditions that are compatible for both LC and MS. These processes involve many experimental factors which need to be simultaneously optimized to obtain maximum sensitivity and resolution at minimum retention time. In this tutorial, the basic concepts of DoE will be explained with focus on practical use of DoE. Three case studies optimized with DoE for liquid chromatography tandem mass spectrometry (LC-MS/MS) quantitative assays will then be presented.
实验设计（DoE）是优化利用液相色谱-串联质谱（LC-MS/MS）的定量生物分析方法的宝贵工具。液相色谱-质谱联用（LC-MS）由几个过程组成，包括液体引入和分析物电离。目标是将分析物从大气压转移到真空，并维持与LC和MS都兼容的条件。这些过程涉及许多实验因素，需要同时进行优化，以便在最短保留时间内获得最大灵敏度和分辨率。在本教程中，将解释DoE的基本概念，并重点关注DoE的实际应用。随后将介绍三个通过DoE优化用于液相色谱串联质谱（LC-MS/MS）定量分析的案例研究。
