Irfan Muhammad, Shagufta Hafiza Arslan, Rasheed Muhammad Usman, Khan Samreen Gul, Aslam Muhammad Mahran, Chen Huanwen
The Jiangxi Province Key Laboratory for Diagnosis, Treatment, and Rehabilitation of Cancer in Chinese Medicine, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi, China.
School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi, China.
Mass Spectrom Rev. 2025 Aug 20. doi: 10.1002/mas.70004.
Mass spectrometry (MS) is a widely recognized analytical technique that can be applied to both large and small molecules and is a potent analytical tool that advances both basic science and practical applications in and out of the lab. MS has become a desirable analytical technique in clinical and bioanalytical labs with its high sensitivity, broad applicability, and throughput benefits. Numerous MS approaches, combined with various separation methods including gas and liquid chromatography, have been proposed to examine intricate matrices. Nevertheless, this technique could be limited by the labor-intensive and drawn-out sample preparation needed before MS analysis. By enabling the direct MS analysis of complex materials under ambient settings with little to no sample pretreatment, AIMS has cut down on both analysis time and expense. Nonetheless, these methods' speed and simplicity make them appropriate for use in critical care and point-of-care testing. Because of this, ambient ionization mass spectrometry (AIMS) has inevitably appealed to a wide range of scientists. The field of AIMS has grown tremendously since its foundation in the mid-2000s, marked by the emergence of numerous new ion sources, a plethora of fascinating applications, and a discernible increase in interest from various fields of study. Around the world, ambient ionization techniques are becoming more and more widespread in laboratories as the science advances yearly. This review focuses on the recent bioanalytical applications of AIMS in fields such as clinics, drugs, forensics, food, and environment, with a specific emphasis on the research reported in manuscripts published during the last 5 years. Although certain methodological developments are mentioned in this review, its primary goal is to demonstrate the practical applications of AIMS in bioanalytical fields. A timely overview of AIMS-based procedures and their application in various sample situations is what this endeavor seeks to give bioanalytical researchers. Moreover, several cutting-edge biological applications have been highlighted to explain how the AIMS area can develop in the future. In this chapter, readers will be provided with a quick overview of the main AIMS methodologies and their recent applied use cases.
质谱分析法（MS）是一种广为人知的分析技术，可应用于大分子和小分子，是一种强大的分析工具，推动了实验室内外的基础科学和实际应用。由于其高灵敏度、广泛适用性和高通量优势，MS已成为临床和生物分析实验室中理想的分析技术。人们提出了许多MS方法，并结合包括气相色谱和液相色谱在内的各种分离方法来检测复杂基质。然而，该技术可能会受到MS分析前所需的劳动密集型和耗时的样品制备的限制。通过在几乎无需样品预处理的环境条件下对复杂材料进行直接MS分析，常压电离质谱分析法（AIMS）减少了分析时间和费用。尽管如此，这些方法的速度和简便性使其适用于重症监护和即时检测。因此，常压电离质谱分析法（AIMS）不可避免地吸引了众多科学家。自21世纪中叶创立以来，AIMS领域发展迅速，其标志是出现了众多新的离子源、大量引人入胜的应用以及各研究领域兴趣的显著增加。随着每年科学的进步，常压电离技术在世界各地的实验室中越来越普遍。本综述重点关注AIMS在临床、药物、法医、食品和环境等领域的最新生物分析应用，特别强调过去5年发表的手稿中报道的研究。尽管本综述提到了某些方法学的发展，但其主要目的是展示AIMS在生物分析领域的实际应用。这项工作旨在为生物分析研究人员提供基于AIMS的程序及其在各种样品情况下应用的及时概述。此外，还强调了一些前沿的生物学应用，以解释AIMS领域未来如何发展。在本章中，将为读者快速概述主要的AIMS方法及其最近的应用案例。
