Toxicology-Microbiology and Environmental Health Laboratory (RL 17ES06), Faculty of Sciences, University of Sfax, 3000, Sfax, Tunisia.
Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, Università di, 98168, Messina, Viale Annunziata, Italy.
Chem Biodivers. 2021 Jun;18(6):e2100185. doi: 10.1002/cbdv.202100185. Epub 2021 May 11.
The genus Rumex (Polygonaceae) is distributed worldwide and the different species belonging to it are used in traditional medicine. The present study aimed at the evaluation of the phytochemical profile and the biochemical properties of methanolic extracts from different parts (roots, stems, and leaves) of Rumex roseus, a wild local Tunisian plant traditionally used as food. The phytochemical analysis on the extracts was performed using standard colorimetric procedures, HPLC-DAD, and HPLC-DAD-ESI-MS; then, several in vitro cell-free assays have been used to estimate their antioxidant/free radical scavenging capability (TAC-PM, DPPH, TEAC, FRAP, ORAC, SOD-like activity, and HOCl-induced albumin degradation). Additionally, anti-inflammatory effect of these extracts was evaluated in an in vitro model of acute intestinal inflammation in differentiated Caco-2 cells. The results showed that the methanolic extracts from stems and, especially, leaves contain substantial amounts of flavones (apigenin and luteolin, together with their derivatives), while the extract from roots is characterized by the presence of tannins and quinic acid derivatives. All the extracts appeared endowed with excellent antioxidant/free radical scavenging properties. In particular, the extract from roots was characterized by a remarkable activity, probably due to its different and peculiar polyphenolic composition. Furthermore, both Rumex roseus roots and stems extracts demonstrated an anti-inflammatory effect in intestinal epithelial cells, reducing TNF-α-induced gene expression of IL-6 and IL-8. In conclusion, R. roseus methanolic extracts have shown to be potential sources of bioactive compounds to be used in the prevention and treatment of pathologies related to oxidative stress and inflammation.
该属酸模（蓼科）分布于世界各地，属于不同的物种被用于传统医学。本研究旨在评估植物化学特征和生化特性的甲醇提取物从不同部位（根，茎和叶）的酸模玫瑰，一个野生的当地突尼斯植物传统上用作食物。在提取物上的植物化学分析采用标准比色法，HPLC-DAD 和 HPLC-DAD-ESI-MS；然后，几种体外无细胞试验已被用于估计他们的抗氧化/自由基清除能力（TAC-PM，DPPH，TEAC，FRAP，ORAC，SOD 样活性和 HOCl 诱导的白蛋白降解）。此外，这些提取物的抗炎作用进行了评价在体外模型的急性肠道炎症分化的 Caco-2 细胞。结果表明，从茎和特别是叶子的甲醇提取物含有大量的类黄酮（芹菜素和木犀草素，与它们的衍生物），而提取物的根是由存在的单宁和奎宁酸衍生物。所有的提取物表现出良好的抗氧化/自由基清除性能。特别是，从根的提取物的特点是一个显著的活动，可能是由于其不同的和独特的多酚组成。此外，玫瑰酸模根和茎提取物表现出抗炎作用在肠上皮细胞，减少 TNF-α 诱导的 IL-6 和 IL-8 的基因表达。总之，R.玫瑰甲醇提取物已被证明是生物活性化合物的潜在来源，可用于预防和治疗与氧化应激和炎症相关的疾病。
