He Jinxin, Zheng Yan, Chen Xiaorong, Li Na, Tang Fang, Huo Nairui, Wang Jianzhong, Gu Shaopeng
College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030810, PR China.
Anal Methods. 2021 Apr 14;13(14):1740-1746. doi: 10.1039/d1ay00133g. Epub 2021 Mar 23.
Mebendazole (MBZ), a synthetic benzimidazole, is most widely used for the treatment of intestinal helminthiasis. In the present study, a hapten mimicking the MBZ structure was designed by introducing propanoic acid and coupling to carrier proteins by the active ester method to immunize New Zealand rabbits. A sensitive enzyme-linked immunosorbent assay (ELISA) was developed for the analysis of MBZ in food samples. The rabbit IgG based ELISA had a half-maximum inhibition concentration (IC) of 7.41 ng mL, with a limit of detection of 0.27 ng mL. The ELISA showed negligible cross reactivity (<1%) with structural analogs, including hydroxy-MBZ, albendazole, oxfendazole, fenbendazole, flubendazole and oxfendazole, except the value of 90.5% for amino-MBZ. The average recoveries of MBZ spiked in chicken and mutton muscle samples by the assay ranged from 84.31% to 106.28% and agreed well with those of high-performance liquid chromatography. The above results indicated that the generated anti-MBZ IgG-based ELISA showed promise in routine screening analysis of MBZ residues in food samples.
甲苯咪唑（MBZ）是一种合成苯并咪唑，被广泛用于治疗肠道蠕虫病。在本研究中，通过引入丙酸设计了一种模拟MBZ结构的半抗原，并采用活性酯法将其与载体蛋白偶联，用于免疫新西兰兔。开发了一种灵敏的酶联免疫吸附测定法（ELISA）用于分析食品样品中的MBZ。基于兔IgG的ELISA的半数抑制浓度（IC）为7.41 ng/mL，检测限为0.27 ng/mL。该ELISA与结构类似物（包括羟基-MBZ、阿苯达唑、奥芬达唑、芬苯达唑、氟苯达唑和奥芬达唑）的交叉反应可忽略不计（<1%），但氨基-MBZ的值为90.5%。该测定法对鸡肉和羊肉肌肉样品中添加的MBZ的平均回收率在84.31%至106.28%之间，与高效液相色谱法的回收率吻合良好。上述结果表明，所产生的基于抗MBZ IgG的ELISA在食品样品中MBZ残留的常规筛选分析中显示出应用前景。
